Recombinant Anti-RAGE antibody [EPR12206] (ab172473)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12206] to RAGE
- Suitable for: WB
- Reacts with: Mouse, Rat
Related conjugates and formulations
Overview
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Product name
Anti-RAGE antibody [EPR12206]
See all RAGE primary antibodies -
Description
Rabbit monoclonal [EPR12206] to RAGE -
Host species
Rabbit -
Specificity
Recent tests in our laboratory showed that the antibody detects the band of interest in tissue lysates, but it did not detect the protein in cell lysate.
RAGE is typically expressed at low levels under normal physiological conditions in majority of tissues except normal lung tissue. When testing other tissues, please use lung tissue as a positive control.
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Tested applications
Suitable for: WBmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat
Does not react with: Human -
Immunogen
Synthetic peptide within Human RAGE aa 350 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q15109 -
Positive control
- Mouse lung, Rat lung.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12206 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab172473 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 42 kDa.
We recommend ab181369 for human samples. |
Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 42 kDa. We recommend ab181369 for human samples. |
Target
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Function
Mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF-alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling (By similarity). Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space. -
Tissue specificity
Endothelial cells. -
Sequence similarities
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 11596 Mouse
- Entrez Gene: 81722 Rat
- SwissProt: Q62151 Mouse
- SwissProt: Q63495 Rat
- Unigene: 3383 Mouse
- Unigene: 9829 Rat
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Alternative names
- Advanced glycosylation end product-specific receptor antibody
- Ager antibody
- DAMA 358M23.4 antibody
see all
Images
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All lanes : Anti-RAGE antibody [EPR12206] (ab172473) at 1/1000 dilution
Lane 1 : Mouse lung lysates
Lane 2 : Mouse brain lysates
Lane 3 : Mouse kidney lysates
Lane 4 : Mouse heart lysates
Lane 5 : Mouse liver lysates
Lane 6 : Mouse spleen lysates
Lane 7 : Rat lung lysates
Lane 8 : Rat brain lysates with 5% NFDM/TBST
Lane 9 : Rat kidney lysates
Lane 10 : Rat heart lysates
Lane 11 : Rat spleen lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 42 kDa
Observed band size: 43 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time:
Lane 1 to 6: 3 second
Lane 7 to 11: 20 secondsThe expression profile and molecular mass observed is consistent with what has been described in the literature (PMID: 16315007; 18355449; 18245812)
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab172473 has been referenced in 6 publications.
- Chen R et al. HMGB1 as a potential biomarker and therapeutic target for severe COVID-19. Heliyon 6:e05672 (2020). PubMed: 33313438
- Tsitsilashvili E et al. Myelin basic protein charge isomers change macrophage polarization. J Inflamm Res 12:25-33 (2019). PubMed: 30774410
- Ryan D et al. Targeting AXL and RAGE to prevent geminin overexpression-induced triple-negative breast cancer metastasis. Sci Rep 9:19150 (2019). PubMed: 31844158
- Ananthula S et al. Geminin overexpression-dependent recruitment and crosstalk with mesenchymal stem cells enhance aggressiveness in triple negative breast cancers. Oncotarget 7:20869-89 (2016). PubMed: 26989079
- Gu JJ et al. Recombinant human soluble thrombomodulin protects against brain injury in a CVST rat model, via downregulation of the HMGB1-RAGE axis. Mol Med Rep 14:5217-5222 (2016). PubMed: 27840921
- Wang XW et al. Small interfering RNA targeting receptor for advanced glycation end products suppresses the generation of proinflammatory cytokines. Exp Ther Med 10:584-590 (2015). PubMed: 26622358