Key features and details
- Rabbit polyclonal to RAGEF2
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-RAGEF2 antibody
DescriptionRabbit polyclonal to RAGEF2
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide corresponding to a region between residues 625 and 675 of human RAGEF2 (NP_057424.2)
- HeLa whole cell lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab86225 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000 - 1/10,000. Predicted molecular weight: 179 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionGuanine nucleotide exchange factor (GEF) for Rap1A, Rap2A and M-Ras GTPases. Does not interact with cAMP.
Tissue specificityIsoform 3 has highest expression levels in the brain, heart, liver, lung and placenta and is barely detectable in skeletal muscle, kidney and pancreas.
Sequence similaritiesContains 1 cyclic nucleotide-binding domain.
Contains 1 N-terminal Ras-GEF domain.
Contains 1 PDZ (DHR) domain.
Contains 1 Ras-associating domain.
Contains 1 Ras-GEF domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationCytoplasm. Cell membrane. Upon binding to M-Ras, it translocates to the plasma membrane.
- Information by UniProt
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All lanes : Anti-RAGEF2 antibody (ab86225) at 0.04 µg
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 179 kDa
Observed band size: 220 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
Detection of human RAGEF2 in Immunoprecipitates of whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab86225 at 3 µg/mg lysate for IP, and at 1 µg/ml for subsequent Western blot.
Detection: Chemiluminescence with an exposure time of 30 seconds.
ab86225 has not yet been referenced specifically in any publications.