Overview

  • Product name

    Anti-RAI14 antibody [EPR8518]
    See all RAI14 primary antibodies
  • Description

    Rabbit monoclonal [EPR8518] to RAI14
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human RAI14 aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control

    • HeLa and 293T cell lysates, Human kidney and Human testis tissues.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR8518
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab137118 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Tissue specificity

      Highly expressed in placenta, muscle, kidney and testis. Moderately expressed in heart, brain, lung, liver and intestine. Isoform 2 is widely expressed and expressed in fetal and adult testes, and spermatozoa.
    • Sequence similarities

      Contains 7 ANK repeats.
    • Cellular localization

      Cytoplasm > cell cortex. Cytoplasm > cytoskeleton. Associated with the cortical actin cytoskeleton structures in terminal web and cell-cell adhesion sites and stress fibers.
    • Information by UniProt
    • Database links

    • Alternative names

      • Ankycorbin antibody
      • Ankyrin repeat and coiled coil structure containing protein antibody
      • Ankyrin repeat and coiled-coil structure-containing protein antibody
      • DKFZp564G013 antibody
      • KIAA1334 antibody
      • NORPEG antibody
      • Novel retinal pigment epithelial cell protein antibody
      • RAI13 antibody
      • RAI14 antibody
      • RAI14_HUMAN antibody
      • Retinoic acid induced 14 antibody
      • Retinoic acid induced protein 14 antibody
      • Retinoic acid-induced protein 14 antibody
      see all

    Images

    • All lanes : Anti-RAI14 antibody [EPR8518] (ab137118) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 110 kDa
      Observed band size: 110 kDa

    • Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling RAI14 with ab137118 antibody at a dilution of 1/100.
    • Immunohistochemical analysis of paraffin embedded Human testis tissue labelling RAI14 with ab137118 antibody at a dilution of 1/100.

    References

    This product has been referenced in:

    • He XY  et al. High Expression of Retinoic Acid Induced 14 (RAI14) in Gastric Cancer and Its Prognostic Value. Med Sci Monit 24:2244-2251 (2018). Read more (PubMed: 29654694) »
    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab137118.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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