Product nameAnti-RAI2 antibody
See all RAI2 primary antibodies
DescriptionRabbit polyclonal to RAI2
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
- ICC/IF: HEK-293 cells. IHC-P: Human cerebral cortex tissue. WB: RT4 and U-251 MG whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab247100 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.4 µg/ml. Predicted molecular weight: 57 kDa.|
|IHC-P||1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100
RelevanceThe specific function of RAI2 (Retinoic acid induced 2) has not yet been determined. However, it is suggested that it may have a role in development since retinoic acid is involved in vertebrate anteroposterior axis formation and cellular differentiation, and has been shown to modulate gene expression controlling early embryonic development.
- RAI 2 antibody
- Retinoic acid induced 2 antibody
- Retinoic acid induced protein 2 antibody
Formalin-fixed, paraffin-embedded human cerebral cortex tissue stained for RAI2 using ab247100 at 1/500 dilution in immunohistochemical analysis.
All lanes : Anti-RAI2 antibody (ab247100) at 0.4 µg/ml
Lane 1 : RT4 (human urinary bladder cancer cell line) whole cell lysate
Lane 2 : U-251 MG (human brain glioma cell line) whole cell lysate
Predicted band size: 57 kDa
PFA-fixed, Triton X-100 permeabilized HEK-293 (human epithelial cell line from embryonic kidney) cells stained for RAI2 (green) using ab247100 at 4μg/ml in ICC/IF.
ab247100 has not yet been referenced specifically in any publications.