Product nameAnti-RAIDD antibody [EPR1640Y]
See all RAIDD primary antibodies
DescriptionRabbit monoclonal [EPR1640Y] to RAIDD
Tested applicationsSuitable for: WB, IP, ICC, Flow Cyt, IHC-Pmore details
Species reactivityReacts with: Human
Synthetic peptide within Human RAIDD (C terminal). The exact sequence is proprietary.
- HeLa cell lysate and Human heart muscle tissue.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.5% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab52621 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).|
|ICC||1/100 - 1/250.|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionApoptotic adaptor molecule specific for caspase-2 and FASL/TNF receptor-interacting protein RIP. In the presence of RIP and TRADD, CRADD recruits caspase-2 to the TNFR-1 signalling complex.
Tissue specificityConstitutively expressed in most tissues, with particularly high expression in adult heart, testis, liver, skeletal muscle, fetal liver and kidney.
Sequence similaritiesContains 1 CARD domain.
Contains 1 death domain.
DomainContains a death domain involved in the binding of RIP protein.
The CARD domain mediates the interaction with caspase-2.
- Information by UniProt
- CASP2 and RIPK1 domain containing adaptor with death domain antibody
- Caspase and RIP adapter with death domain antibody
- Caspase and RIP adaptor with death domain antibody
Anti-RAIDD antibody [EPR1640Y] (ab52621) at 1/1000 dilution + HeLa cell lysate at 10 µg
goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 23 kDa
Observed band size: 23 kDa
ab52621, at a 1/50 dilution, staining Human RAIDD in heart muscle, using Immunohistochemistry, Paraffin embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
This product has been referenced in:
- Cruz VH et al. Loss of Tbk1 kinase activity protects mice from diet-induced metabolic dysfunction. Mol Metab 16:139-149 (2018). Read more (PubMed: 29935921) »
- Di Donato N et al. Mutations in CRADD Result in Reduced Caspase-2-Mediated Neuronal Apoptosis and Cause Megalencephaly with a Rare Lissencephaly Variant. Am J Hum Genet 99:1117-1129 (2016). Read more (PubMed: 27773430) »