Recombinant Anti-RAIDD antibody [EPR1640Y] (ab52621)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1640Y] to RAIDD
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-RAIDD antibody [EPR1640Y]
See all RAIDD primary antibodies -
Description
Rabbit monoclonal [EPR1640Y] to RAIDD -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human RAIDD (C terminal). The exact sequence is proprietary.
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Positive control
- HeLa cell lysate and Human heart muscle tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR1640Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab52621 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Notes |
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WB
1/1000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa). |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Apoptotic adaptor molecule specific for caspase-2 and FASL/TNF receptor-interacting protein RIP. In the presence of RIP and TRADD, CRADD recruits caspase-2 to the TNFR-1 signalling complex. -
Tissue specificity
Constitutively expressed in most tissues, with particularly high expression in adult heart, testis, liver, skeletal muscle, fetal liver and kidney. -
Sequence similarities
Contains 1 CARD domain.
Contains 1 death domain. -
Domain
Contains a death domain involved in the binding of RIP protein.
The CARD domain mediates the interaction with caspase-2. - Information by UniProt
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Database links
- Entrez Gene: 8738 Human
- Omim: 603454 Human
- SwissProt: P78560 Human
- Unigene: 38533 Human
- Unigene: 591016 Human
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Alternative names
- CASP2 and RIPK1 domain containing adaptor with death domain antibody
- Caspase and RIP adapter with death domain antibody
- Caspase and RIP adaptor with death domain antibody
see all
Images
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Anti-RAIDD antibody [EPR1640Y] (ab52621) at 1/1000 dilution + HeLa cell lysate at 10 µg
Secondary
Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 23 kDa
Observed band size: 23 kDa -
ab52621, at a 1/50 dilution, staining Human RAIDD in heart muscle, using Immunohistochemistry, Paraffin embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab52621 has been referenced in 4 publications.
- Cruz VH et al. Loss of Tbk1 kinase activity protects mice from diet-induced metabolic dysfunction. Mol Metab 16:139-149 (2018). PubMed: 29935921
- Di Donato N et al. Mutations in CRADD Result in Reduced Caspase-2-Mediated Neuronal Apoptosis and Cause Megalencephaly with a Rare Lissencephaly Variant. Am J Hum Genet 99:1117-1129 (2016). PubMed: 27773430
- Shen Q et al. Regulation of CRADD-caspase 2 cascade by histone deacetylase 1 in gastric cancer. Am J Transl Res 6:538-47 (2014). PubMed: 25360218
- Jelínek M et al. Caspase-2 is involved in cell death induction by taxanes in breast cancer cells. Cancer Cell Int 13:42 (2013). WB . PubMed: 23672670