Anti-RAIN antibody (ab129820)
Key features and details
- Rabbit polyclonal to RAIN
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-RAIN antibody
See all RAIN primary antibodies -
Description
Rabbit polyclonal to RAIN -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Rat, Rabbit, Horse, Cow, Dog, Human, Pig, Chimpanzee, Macaque monkey, Gorilla, Orangutan -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- This antibody gave a positive signal in Mouse Lung tissue lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab129820 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/250. Detects a band of approximately 115 kDa (predicted molecular weight: 103 kDa).
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Notes |
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WB
1/250. Detects a band of approximately 115 kDa (predicted molecular weight: 103 kDa). |
Target
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Function
May act as effector for Golgi-bound HRAS and other Ras-like proteins. May promote HRAS-mediated transformation. -
Tissue specificity
Highly expressed in heart. Detected at lower levels in placenta and pancreas. -
Sequence similarities
Contains 1 dilute domain.
Contains 1 Ras-associating domain. -
Cellular localization
Cytoplasm > perinuclear region. Golgi apparatus > Golgi stack. Associated with perinuclear vesicles. Is recruited to Golgi stacks by activated HRAS. - Information by UniProt
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Database links
- Entrez Gene: 54922 Human
- Entrez Gene: 69903 Mouse
- Omim: 609623 Human
- SwissProt: Q5U651 Human
- SwissProt: Q3U0S6 Mouse
- Unigene: 233955 Human
- Unigene: 181396 Mouse
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Alternative names
- FLJ20401 antibody
- Rain antibody
- RAIN protein fragment antibody
see all
Images
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Anti-RAIN antibody (ab129820) at 1/250 dilution +
Mouse lung normal tissue lysate - total protein (ab29297) at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 103 kDa
Observed band size: 115 kDa why is the actual band size different from the predicted?
Additional bands at: 23 kDa, 39 kDa, 68 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
The expression profile observed is consistent with what has been described in the literature PMID:15031288. This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab129820 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab129820 has not yet been referenced specifically in any publications.