Tested in peptide ELISA. Antibody detection limit dilution 1/8000.
WB: Use at a concentration of 0.3 µg/ml. Predicted molecular weight: 110 kDa.
WB experiments gave bands at approx 95kDa, 75kDa and 45kDa in Lung Carcinoma lysate after antibody staining. Please note that currently we cannot find an explanation in the literature for the additional bands we observe given the predicted size according to NP_060275. The detected bands were successfully blocked by incubation with the immunizing peptide.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
May act as effector for Golgi-bound HRAS and other Ras-like proteins. May promote HRAS-mediated transformation.
Highly expressed in heart. Detected at lower levels in placenta and pancreas.
Norden PR et al. Cdc42 and k-Ras Control Endothelial Tubulogenesis through Apical Membrane and Cytoskeletal Polarization: Novel Stimulatory Roles for GTPase Effectors, the Small GTPases, Rac2 and Rap1b, and Inhibitory Influence of Arhgap31 and Rasa1. PLoS One11:e0147758 (2016).
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