Key features and details
- Goat polyclonal to RAIN
- Reacts with: Human
- Isotype: IgG
Product nameAnti-RAIN antibody
See all RAIN primary antibodies
Species reactivityReacts with: Human
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
WB: Use at a concentration of 0.3 µg/ml. Predicted molecular weight: 110 kDa.
WB experiments gave bands at approx 95kDa, 75kDa and 45kDa in Lung Carcinoma lysate after antibody staining. Please note that currently we cannot find an explanation in the literature for the additional bands we observe given the predicted size according to NP_060275. The detected bands were successfully blocked by incubation with the immunizing peptide.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionMay act as effector for Golgi-bound HRAS and other Ras-like proteins. May promote HRAS-mediated transformation.
Tissue specificityHighly expressed in heart. Detected at lower levels in placenta and pancreas.
Sequence similaritiesContains 1 dilute domain.
Contains 1 Ras-associating domain.
Cellular localizationCytoplasm > perinuclear region. Golgi apparatus > Golgi stack. Associated with perinuclear vesicles. Is recruited to Golgi stacks by activated HRAS.
- Information by UniProt
- FLJ20401 antibody
- Rain antibody
- RAIN protein fragment antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab21018 has been referenced in 3 publications.
- Liu X et al. Rasip1 controls lymphatic vessel lumen maintenance by regulating endothelial cell junctions. Development 145:N/A (2018). PubMed: 30042182
- Norden PR et al. Cdc42 and k-Ras Control Endothelial Tubulogenesis through Apical Membrane and Cytoskeletal Polarization: Novel Stimulatory Roles for GTPase Effectors, the Small GTPases, Rac2 and Rap1b, and Inhibitory Influence of Arhgap31 and Rasa1. PLoS One 11:e0147758 (2016). PubMed: 26812085
- Xu K et al. Blood vessel tubulogenesis requires Rasip1 regulation of GTPase signaling. Dev Cell 20:526-39 (2011). PubMed: 21396893