Anti-Rainbow Ladder antibody [2C4] (HRP) (ab231759)

Overview

  • Product name

    Anti-Rainbow Ladder antibody [2C4] (HRP)
  • Description

    Mouse monoclonal [2C4] to Rainbow Ladder (HRP)
  • Host species

    Mouse
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Other Immunogen Type corresponding to Rainbow Ladder. Remazol dye stained proteins, prestained multi-color protein markers.

  • General notes

    Ab231759 this monoclonal detects multi-color pre-stained protein markers (specifically Remazol dye stained proteins). It allows the visualisation of pre-stained protein ladders in combination with the bands of interest without needing to merge images or manually mark film (PubMed ID: 27531616). The antibody is conjugated to HRP to permit direct visualisation by chemiluminescence.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Stable for 12 months at -20°C. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.1% Proclin
    Constituents: PBS, 30% Glycerol, 1% BSA
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    2C4
  • Isotype

    IgG1
  • Light chain type

    kappa

Applications

Our Abpromise guarantee covers the use of ab231759 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000.

Images

  • All lanes : Anti-Rainbow Ladder antibody [2C4] (HRP) (ab231759) at 1/5000 dilution

    Lane 1 : Chameleon Marker
    Lane 2 : Thermo Scientific Pageruler Plus
    Lane 3 : All Blue BioRad

    Lysates/proteins at 1 µl per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 30 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab231759 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

References

ab231759 has not yet been referenced specifically in any publications.

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