Product nameAnti-Rainbow Ladder antibody [2C4] (HRP)
DescriptionMouse monoclonal [2C4] to Rainbow Ladder (HRP)
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Species independent
Other Immunogen Type corresponding to Rainbow Ladder. Remazol dye stained proteins, prestained multi-color protein markers.
Ab231759 this monoclonal detects multi-color pre-stained protein markers (specifically Remazol dye stained proteins). It allows the visualisation of pre-stained protein ladders in combination with the bands of interest without needing to merge images or manually mark film (PubMed ID: 27531616). The antibody is conjugated to HRP to permit direct visualisation by chemiluminescence.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Stable for 12 months at -20°C. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.1% Proclin
Constituents: PBS, 30% Glycerol, 1% BSA
Concentration information loading...
PurityProtein G purified
Light chain typekappa
Our Abpromise guarantee covers the use of ab231759 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
All lanes : Anti-Rainbow Ladder antibody [2C4] (HRP) (ab231759) at 1/5000 dilution
Lane 1 : Chameleon Marker
Lane 2 : Thermo Scientific Pageruler Plus
Lane 3 : All Blue BioRad
Lysates/proteins at 1 µl per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 30 seconds
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab231759 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
ab231759 has not yet been referenced specifically in any publications.