Overview

  • Product name
    Raji whole cell lysate
    See all Raji lysates
  • General notes


    Protein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC.

  • Tested applications
    Suitable for: WBmore details

Properties

  • Form
    Liquid
  • Storage instructions
    Store at -80°C. Allow to warm to room temp and agitate gently before aliquotting.
  • Storage buffer
    pH: 7.90
    Constituents: 1% Tergitol-NP40, Potassium chloride, 0.1% Bromophenol blue, 1.2% Beta mercaptoethanol, 2% SDS, HEPES, Sucrose, 10% Glycerol
  • Concentration information loading...
  • Lysate notes
    Protein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC.
  • Research areas
  • Background
    Established in 1963 from the left maxilla of a 12-year-old African boy with Burkitt's lymphoma, it is the first continuous human hematopoietic cell line. It has become an indispensable tool in haematological research and leukaemia and lymphoma studies.

Applications

Our Abpromise guarantee covers the use of ab30124 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Samples need to boiled for 5 minutes and put on ice before use.

References

ab30124 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-4 of 4 Q&A

Answer

Thank you for contacting us.


This protein lysate could contain proteases. That’s why we put protease inhibitor cocktail in the protein lyses buffer.


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Vielen Dank für Ihre Anfrage.

Für ab111915 wurden 20 µg Lysat aufgetragen, für ab66106 waren es10 µg (siehe Datenblatt). Normalerweise sind - je nach Expressionslevel in der Positivkontrolle - 20 bis maximal 50 µg mehr als ausreichend, bei rekombinantem Protein sogar noch weniger.

Wie Sie vielleicht schon gesehen haben, haben wir die entsprechenden Lysate im Katalog:

ab29890: Liver (Human) Tissue Lysate - fetal normal tissue

https://www.abcam.com/index.html?datasheet=29890 (or use the following: https://www.abcam.com/index.html?datasheet=29890).

ab7912: MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate

https://www.abcam.com/index.html?datasheet=7912 (or use the following: https://www.abcam.com/index.html?datasheet=7912).

ab30124: Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate - tumor cell line

https://www.abcam.com/index.html?datasheet=30124 (or use the following: https://www.abcam.com/index.html?datasheet=30124).

Ich wünsche Ihnen viel Erfolg für Ihr Projekt. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

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Answer

Thank you for your enquiry regarding ab52610.

The phosphorylation of PLCG 2 at Tyrosine 1217 needs to be induced to be able to detect this specific modification. As the WB image on the datasheet indicates Raji cell lysate without stimulation will not provide signal for phospho Y1217. It is important to treat the cells with inducer such as pervanadate.

If you need any further assistance in the future, please do not hesitate to contact me.

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Question
Answer

Thank you for contacting Abcam Our protein lysate is called total protein lysate, which means most of the proteins, including membrane and surface proteins, are within the lysate. We don’t really have a cell number to generate 100ug lysate, we can only estimate in this way, assuming 125 million cells as 5 g, and usually there is about 10% lysate, so 500 mg /125million cells, then 100 ug lysate is estimated from 25,000 cells. I have also attached the Abcam protocols book that contains a section on choosing the correct lysis buffer, when trying to capture different parts of the cell. If there is anything else I can help you with, please let me know.  

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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