Anti-RALA antibody (ab236314)
Key features and details
- Rabbit polyclonal to RALA
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RALA antibody
See all RALA primary antibodies -
Description
Rabbit polyclonal to RALA -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Saguinus oedipus -
Immunogen
Recombinant fragment corresponding to Human RALA aa 1 to the C-terminus.
Database link: P11233 -
Positive control
- IHC-P: Human adrenal gland tissue. ICC/IF: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab236314 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/20 - 1/200.
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IHC-P |
1/20 - 1/200.
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Notes |
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ICC/IF
1/20 - 1/200. |
IHC-P
1/20 - 1/200. |
Target
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Function
Multifuntional GTPase involved in a variety of cellular processes including gene expression, cell migration, cell proliferation, oncogenic transformation and membrane trafficking. Accomplishes its multiple functions by interacting with distinct downstream effectors. Acts as a GTP sensor for GTP-dependent exocytosis of dense core vesicles. Plays a role in the early stages of cytokinesis and is required to tether the exocyst to the cytokinetic furrow. The RALA-exocyst complex regulates integrin-dependent membrane raft exocytosis and growth signaling. Key regulator of LPAR1 signaling and competes with ADRBK1 for binding to LPAR1 thus affecting the signaling properties of the receptor. Required for anchorage-independent proliferation of transformed cells. -
Sequence similarities
Belongs to the small GTPase superfamily. Ras family. -
Post-translational
modificationsPrenylation is essential for membrane localization. The geranylgeranylated form and the farnesylated mutant does not undergo alternative prenylation in response to geranylgeranyltransferase I inhibitors (GGTIs) and farnesyltransferase I inhibitors (FTIs). -
Cellular localization
Cell surface. Cell membrane. Cleavage furrow. Midbody. Prior to LPA treatment found predominantly at the cell surface and in the presence of LPA co-localizes with LPAR1 and LPAR2 in the endocytic vesicles. During early cytokinesis localizes at the cleavage furrow membrane. Colocalizes with EXOC2 at the early midbody ring and persists there till maturation of the midbody. - Information by UniProt
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Database links
- Entrez Gene: 5898 Human
- Entrez Gene: 56044 Mouse
- Entrez Gene: 81757 Rat
- Omim: 179550 Human
- SwissProt: P11233 Human
- SwissProt: P63321 Mouse
- SwissProt: P63322 Rat
- Unigene: 6906 Human
see all -
Alternative names
- MGC48949 antibody
- Ral a antibody
- Ral A protein antibody
see all
Images
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Paraffin-embedded human adrenal gland tissue stained for RALA using ab236314 at 1/100 dilution in immunohistochemical analysis.
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4% formaldehyde-fixed, 0.2 Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling RALA using ab236314 (green) at 1/100 dilution in ICC/IF.
Cells were blocked in 10% normal goat serum and incubated with primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor® 488-conjugated goat anti-rabbit IgG (H+L). Counter stained with DAPI (blue).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab236314 has been referenced in 1 publication.
- Fan T et al. Immune and non-immune cell subtypes identify novel targets for prognostic and therapeutic strategy: A study based on intratumoral heterogenicity analysis of multicenter scRNA-seq datasets in lung adenocarcinoma. Front Immunol 13:1046121 (2022). PubMed: 36483553