Recombinant
Anti-RALA antibody [EPR6468] (ab126627)
- Datasheet
- Specific References (2)
- Protocols
Overview
-
Product nameAnti-RALA antibody [EPR6468]
See all RALA primary antibodies -
DescriptionRabbit monoclonal [EPR6468] to RALA
-
Host speciesRabbit
-
Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
Unsuitable for: ICC/IF or IP -
Species reactivityReacts with: Human
-
Immunogen
Synthetic peptide within Human RALA aa 100-200. The exact sequence is proprietary.
-
Positive control
- MCF7, MOLT4, BxPC3 and HepG2 cell lysates; Human breast carcinoma tissue.
-
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
-
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
-
ClonalityMonoclonal
-
Clone numberEPR6468
-
IsotypeIgG
-
Research areas
Associated products
-
Compatible Secondaries
-
Positive Controls
Applications
Our Abpromise guarantee covers the use of ab126627 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 24 kDa. | |
| IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| Flow Cyt | 1/1000 - 1/10000. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|
Application notes
Is unsuitable for ICC/IF or IP.
Target
-
FunctionMultifuntional GTPase involved in a variety of cellular processes including gene expression, cell migration, cell proliferation, oncogenic transformation and membrane trafficking. Accomplishes its multiple functions by interacting with distinct downstream effectors. Acts as a GTP sensor for GTP-dependent exocytosis of dense core vesicles. Plays a role in the early stages of cytokinesis and is required to tether the exocyst to the cytokinetic furrow. The RALA-exocyst complex regulates integrin-dependent membrane raft exocytosis and growth signaling. Key regulator of LPAR1 signaling and competes with ADRBK1 for binding to LPAR1 thus affecting the signaling properties of the receptor. Required for anchorage-independent proliferation of transformed cells.
-
Sequence similaritiesBelongs to the small GTPase superfamily. Ras family.
-
Post-translational
modificationsPrenylation is essential for membrane localization. The geranylgeranylated form and the farnesylated mutant does not undergo alternative prenylation in response to geranylgeranyltransferase I inhibitors (GGTIs) and farnesyltransferase I inhibitors (FTIs). -
Cellular localizationCell surface. Cell membrane. Cleavage furrow. Midbody. Prior to LPA treatment found predominantly at the cell surface and in the presence of LPA co-localizes with LPAR1 and LPAR2 in the endocytic vesicles. During early cytokinesis localizes at the cleavage furrow membrane. Colocalizes with EXOC2 at the early midbody ring and persists there till maturation of the midbody.
- Information by UniProt
-
Database links
- Entrez Gene: 5898 Human
- Omim: 179550 Human
- SwissProt: P11233 Human
- Unigene: 6906 Human
-
Alternative names
- MGC48949 antibody
- Ral a antibody
- Ral A protein antibody
see all
Images
-
![Flow Cytometry - Anti-RALA antibody [EPR6468] (ab126627)](https://www.abcam.com/ps/products/126/ab126627/Images/ab126627-3-ab126627FC.jpg)
Flow Cytometry - Anti-RALA antibody [EPR6468] (ab126627)Overlay histogram showing MCF7 cells stained with ab126627 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab126627, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions. -
![Western blot - Anti-RALA antibody [EPR6468] (ab126627)](https://www.abcam.com/ps/datasheet/images/126/ab126627/RALA-Primary-antibodies-ab126627-1.jpg)
Western blot - Anti-RALA antibody [EPR6468] (ab126627)All lanes : Anti-RALA antibody [EPR6468] (ab126627) at 1/1000 dilution
Lane 1 : MCF7 cell lysate
Lane 2 : MOLT4 cell lysate
Lane 3 : BxPC3 cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 24 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RALA antibody [EPR6468] (ab126627)](https://www.abcam.com/ps/datasheet/images/126/ab126627/RALA-Primary-antibodies-ab126627-2.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RALA antibody [EPR6468] (ab126627)ab126627, at 1/100, staining RALA in formalin fixed, paraffin embedded Human breast carcinoma tissue by Immunohistochemistry.
References
This product has been referenced in:
- Omsland M et al. Tunneling nanotube (TNT) formation is downregulated by cytarabine and NF-?B inhibition in acute myeloid leukemia (AML). Oncotarget 8:7946-7963 (2017). Read more (PubMed: 27974700) »
- Lianoglou S et al. Ubiquitously transcribed genes use alternative polyadenylation to achieve tissue-specific expression. Genes Dev 27:2380-96 (2013). WB ; Human . Read more (PubMed: 24145798) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"