Key features and details
- Rabbit polyclonal to RALA+RALB
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-RALA+RALB antibody
DescriptionRabbit polyclonal to RALA+RALB
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Xenopus laevis, Zebrafish, Cynomolgus monkey, Orangutan
Synthetic peptide corresponding to Human RALA+RALB aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- ab42532 gave a positive result in the following Whole Cell Lysates HepG2 MDA MB 361
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab42532 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 24 kDa).|
RelevanceRALA, resorcylic acid lactone alpha, is a low molecular weight GTP binding protein belonging to the RAS family of onco proteins. RALA has a 50% amino acid homology with RAS and an 85% homology with RALB. GTP binding proteins mediate the transmembrane signaling initiated by the occupancy of certain cell surface receptors.
Cellular localizationCell Membrane
- DRal antibody
- Ral antibody
- RalA protein antibody
All lanes : Anti-RALA+RALB antibody (ab42532) at 1 µg/ml
Lane 1 : HepG2 whole cell lysate (ab7900)
Lane 2 : MDA MB 361 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 24 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?
ab42532 has not yet been referenced specifically in any publications.