Overview

  • Product name

    Anti-RALB antibody [EPR6471]
    See all RALB primary antibodies
  • Description

    Rabbit monoclonal [EPR6471] to RALB
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues near the C-terminus of Human RALB. (Uniprot: P11234)

  • Positive control

    • HepG2, 293T and NIH/3T3 cell lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab129077 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 24 kDa (predicted molecular weight: 23 kDa).
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Function

      Multifunctional GTPase involved in a variety of cellular processes including gene expression, cell migration, cell proliferation, oncogenic transformation and membrane trafficking. Accomplishes its multiple functions by interacting with distinct downstream effectors. Acts as a GTP sensor for GTP-dependent exocytosis of dense core vesicles. Required both to stabilize the assembly of the exocyst complex and to localize functional exocyst complexes to the leading edge of migrating cells. Plays a role in the late stages of cytokinesis and is required for the abscission of the bridge joining the sister cells emerging from mitosis. Required for suppression of apoptosis.
    • Sequence similarities

      Belongs to the small GTPase superfamily. Ras family.
    • Post-translational
      modifications

      Prenylation is essential for membrane localization. The geranylgeranylated form and the farnesylated mutant does not undergo alternative prenylation in response to geranylgeranyltransferase I inhibitors (GGTIs) and farnesyltransferase I inhibitors (FTIs).
    • Cellular localization

      Cell membrane. During late cytokinesis localizes at the midbody.
    • Information by UniProt
    • Database links

    • Alternative names

      • 5730472O18Rik antibody
      • dRalb antibody
      • GTP binding protein antibody
      • Ralb antibody
      • RALB_HUMAN antibody
      • RAS like protein B antibody
      • RAS like proto oncogene B antibody
      • Ras related GTP binding protein B antibody
      • Ras-related protein Ral-B antibody
      • v ral simian leukemia viral oncogene homolog B (ras related GTP binding protein) antibody
      • v ral simian leukemia viral oncogene homolog B (ras related) antibody
      • V ral simian leukemia viral oncogene homolog B antibody
      see all

    Images

    • All lanes : Anti-RALB antibody [EPR6471] (ab129077) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : NIH/3T3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-rabbit HRP

      Predicted band size: 23 kDa
      Observed band size: 24 kDa
      why is the actual band size different from the predicted?

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab129077 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab129077.
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