Anti-RALBP1 antibody (ab231900)
Key features and details
- Rabbit polyclonal to RALBP1
- Suitable for: WB, IHC-P
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-RALBP1 antibody
See all RALBP1 primary antibodies -
Description
Rabbit polyclonal to RALBP1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Rat, Human -
Immunogen
Recombinant fragment (His-tag) corresponding to Mouse RALBP1 aa 150-400. N-terminal tag. (Expressed in E.coli).
Database link: Q62172 -
Positive control
- WB: Recombinant mouse RALBP1 and mouse serum. IHC-P: Mouse liver tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 44.12% PBS, 55.77% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab231900 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 75 kDa.
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IHC-P | (1) |
Use a concentration of 5 - 20 µg/ml.
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Notes |
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WB
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 75 kDa. |
IHC-P
Use a concentration of 5 - 20 µg/ml. |
Target
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Function
Can activate specifically hydrolysis of GTP bound to RAC1 and CDC42, but not RALA. Mediates ATP-dependent transport of S-(2,4-dinitrophenyl)-glutathione (DNP-SG) and doxorubicin (DOX) and is the major ATP-dependent transporter of glutathione conjugates of electrophiles (GS-E) and DOX in erythrocytes. Can catalyze transport of glutathione conjugates and xenobiotics, and may contribute to the multidrug resistance phenomenon. Serves as a scaffold protein that brings together proteins forming an endocytotic complex during interphase and also with CDK1 to switch off endocytosis, One of its substrates would be EPN1/Epsin. -
Tissue specificity
Expressed ubiquitously but at low levels. Shows a strong expression in the erythrocytes. -
Sequence similarities
Contains 1 Rho-GAP domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 10928 Human
- Entrez Gene: 19765 Mouse
- Entrez Gene: 84014 Rat
- Omim: 605801 Human
- SwissProt: Q15311 Human
- SwissProt: Q62172 Mouse
- SwissProt: Q62796 Rat
- Unigene: 528993 Human
see all -
Alternative names
- RLIP1 antibody
- 76 kDa Ral-interacting protein antibody
- 76-kDa Ral-interacting protein antibody
see all
Images
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Formalin-fixed, paraffin-embedded mouse liver tissue stained for RALBP1 using ab231900 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Anti-RALBP1 antibody (ab231900) at 1 µg/ml + Recombinant mouse RALBP1 protein
Developed using the ECL technique.
Predicted band size: 75 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted? -
Anti-RALBP1 antibody (ab231900) at 1 µg/ml + Mouse serum
Developed using the ECL technique.
Predicted band size: 75 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab231900 has not yet been referenced specifically in any publications.