Anti-RALBP1 antibody (ab33446)


  • Product name
  • Description
    Rabbit polyclonal to RALBP1
  • Host species
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 600 to the C-terminus of Human RALBP1.

    (Peptide available as ab33445.)

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: Jurkat, HEK293, Hela and A431. The data for Hela and A431 is not shown.



Our Abpromise guarantee covers the use of ab33446 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 76 kDa).


  • Function
    Can activate specifically hydrolysis of GTP bound to RAC1 and CDC42, but not RALA. Mediates ATP-dependent transport of S-(2,4-dinitrophenyl)-glutathione (DNP-SG) and doxorubicin (DOX) and is the major ATP-dependent transporter of glutathione conjugates of electrophiles (GS-E) and DOX in erythrocytes. Can catalyze transport of glutathione conjugates and xenobiotics, and may contribute to the multidrug resistance phenomenon. Serves as a scaffold protein that brings together proteins forming an endocytotic complex during interphase and also with CDK1 to switch off endocytosis, One of its substrates would be EPN1/Epsin.
  • Tissue specificity
    Expressed ubiquitously but at low levels. Shows a strong expression in the erythrocytes.
  • Sequence similarities
    Contains 1 Rho-GAP domain.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • RLIP1 antibody
    • 76 kDa Ral-interacting protein antibody
    • 76-kDa Ral-interacting protein antibody
    • Dinitrophenyl S-glutathione ATPase antibody
    • DNP-SG ATPase antibody
    • Ral-interacting protein 1 antibody
    • Ral-interacting protein 1, 76-KD antibody
    • RalA-binding protein 1 antibody
    • RalBP1 antibody
    • RBP1_HUMAN antibody
    • RIP1 antibody
    • RLIP1 antibody
    • RLIP76 antibody
    see all


  • All lanes : Anti-RALBP1 antibody (ab33446) at 1 µg/ml

    Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 76 kDa
    Observed band size: 90 kDa (why is the actual band size different from the predicted?)

    The band seen at 90 kDa is consistent with the banding pattern observed for other commercially available antibodies to RIP1.
  • ICC/IF image of ab33446 stained human HEK 293 cells. The cells were methanol fixed (5 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab33446, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).


ab33446 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Human Cell lysate - whole cell (HEK293)
Loading amount
20 µg
Gel Running Conditions
Non-reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Feb 01 2013

Thank you for contacting us. We do have positive controls available for these antibodies. The Jurkat lysate catalog number is ab7899. This should also be an appropriate control for the anti-RIP3 antibody, as we do not have the HEK293 transfected cells ...

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Thank you for your enquiry. Your search using the keyword "RLIP76" pulled out two RIP1 antibody datasheets (ab33446, ab56815). Although the product name is different, these products are RLIP76 antibodies. If you click on the [Alternative names] lin...

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