Key features and details
- Rabbit polyclonal to RALBP1 - C-terminal
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-RALBP1 antibody - C-terminal
See all RALBP1 primary antibodies
DescriptionRabbit polyclonal to RALBP1 - C-terminal
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Common marmoset, Orangutan
- 293T and Jurkat whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab191167 was affinity purified using an epitope specific to RALBP1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab191167 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 76 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionCan activate specifically hydrolysis of GTP bound to RAC1 and CDC42, but not RALA. Mediates ATP-dependent transport of S-(2,4-dinitrophenyl)-glutathione (DNP-SG) and doxorubicin (DOX) and is the major ATP-dependent transporter of glutathione conjugates of electrophiles (GS-E) and DOX in erythrocytes. Can catalyze transport of glutathione conjugates and xenobiotics, and may contribute to the multidrug resistance phenomenon. Serves as a scaffold protein that brings together proteins forming an endocytotic complex during interphase and also with CDK1 to switch off endocytosis, One of its substrates would be EPN1/Epsin.
Tissue specificityExpressed ubiquitously but at low levels. Shows a strong expression in the erythrocytes.
Sequence similaritiesContains 1 Rho-GAP domain.
- Information by UniProt
- RLIP1 antibody
- 76 kDa Ral-interacting protein antibody
- 76-kDa Ral-interacting protein antibody
All lanes : Anti-RALBP1 antibody - C-terminal (ab191167) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 76 kDa
Exposure time: 30 seconds
Lysates prepared using NETN lysis buffer.
Detection of RALBP1 in immunoprecipitates of 293T whole cell lysate prepared using NETN lysis buffer (1 mg for IP, 20% of IP loaded) using ab191167 at 6 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection (Lane 1). Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 30 seconds.
ab191167 has not yet been referenced specifically in any publications.