The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 100 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Stimulates the dissociation of GDP from the Ras related RalA and RalB GTPases which allows GTP binding and activation of the GTPases. Interacts and acts as an effector molecule for R Ras, H Ras, K Ras, and Rap (from SwissProt).