Overview

  • Product name

  • Description

    Rabbit polyclonal to RALY
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human RALY aa 254-306.
    Sequence:

    PAPQENTTSEAGLPQGEARTRDDGDEEGLLTHSEEELEHSQDTDADDGAL Q


    Database link: Q9UKM9

  • Positive control

    • WB: A549, HepG2 and Jurkat whole cell lysate (ab7899). IHC-P: Human prostate cancer and pancreatic cancer tissue. ICC/IF: HeLa cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.4
    Preservative: 0.03% Proclin
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purity >95%.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab236610 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/5000. Predicted molecular weight: 32 kDa.
IHC-P 1/500 - 1/1000.
ICC/IF 1/50 - 1/200.

Target

  • Function

    Probable-RNA binding protein. Could be a heterogeneous nuclear ribonucleoprotein (hnRNP). May be involved in pre-mRNA splicing.
  • Tissue specificity

    Expressed in heart, brain, lung, liver, skeletal muscle, kidney and pancreas. Weakly expressed in placenta.
  • Sequence similarities

    Belongs to the RRM HNRPC family. RALY subfamily.
    Contains 1 RRM (RNA recognition motif) domain.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Autoantigen p542 antibody
    • Heterogeneous nuclear ribonucleoprotein C-like 2 antibody
    • hnRNP associated with lethal yellow protein homolog antibody
    • hnRNP core protein C-like 2 antibody
    • MGC117312 antibody
    • P542 antibody
    • RALY antibody
    • RALY_HUMAN antibody
    • RNA binding protein (autoantigenic hnRNP associated with lethal yellow) antibody
    • RNA binding protein (autoantigenic) antibody
    • RNA binding protein autoantigenic (hnRNP associated with lethal yellow homolog (mouse)) antibody
    • RNA-binding protein Raly antibody
    see all

Images

  • All lanes : Anti-RALY antibody (ab236610) at 1/500 dilution

    Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate
    Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

    Secondary
    All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution

    Predicted band size: 32 kDa

  • Paraffin-embedded human prostate cancer tissue stained for RALY using ab236610 at 1/500 dilution in immunohistochemical analysis.

    After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Paraffin-embedded human pancreatic cancer tissue stained for RALY using ab236610 at 1/500 dilution in immunohistochemical analysis.

    After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for RALY  using ab236610 at a dilution of 1/167 in ICC/IF.

    The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the primary antibody overnight at 4°C. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L).

References

ab236610 has not yet been referenced specifically in any publications.

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