Recombinant
RabMAb

Anti-RALY antibody [EPR10121] (ab170105)

Overview

  • Product name
    Anti-RALY antibody [EPR10121]
    See all RALY primary antibodies
  • Description
    Rabbit monoclonal [EPR10121] to RALY
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, IPmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to residues in Human RALY (UniProt: Q9UKM9).

  • Positive control
    • HeLa, HepG2, Jurkat and A549 lysates; Human brain and heart tissues; HepG2 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR10121
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab170105 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 32 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Use of an HRP/AP polymerized secondary antibody recommended.
ICC/IF 1/250 - 1/500.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function
      Probable-RNA binding protein. Could be a heterogeneous nuclear ribonucleoprotein (hnRNP). May be involved in pre-mRNA splicing.
    • Tissue specificity
      Expressed in heart, brain, lung, liver, skeletal muscle, kidney and pancreas. Weakly expressed in placenta.
    • Sequence similarities
      Belongs to the RRM HNRPC family. RALY subfamily.
      Contains 1 RRM (RNA recognition motif) domain.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Autoantigen p542 antibody
      • Heterogeneous nuclear ribonucleoprotein C-like 2 antibody
      • hnRNP associated with lethal yellow protein homolog antibody
      • hnRNP core protein C-like 2 antibody
      • MGC117312 antibody
      • P542 antibody
      • RALY antibody
      • RALY_HUMAN antibody
      • RNA binding protein (autoantigenic hnRNP associated with lethal yellow) antibody
      • RNA binding protein (autoantigenic) antibody
      • RNA binding protein autoantigenic (hnRNP associated with lethal yellow homolog (mouse)) antibody
      • RNA-binding protein Raly antibody
      see all

    Images

    • All lanes : Anti-RALY antibody [EPR10121] (ab170105) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : A549 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 32 kDa

    • Immunohistochemical analysis of paraffin embedded Human brain tissue labeling RALY with ab170105 at a 1/100 dilution.
    • Immunohistochemical analysis of paraffin embedded Human heart tissue labeling RALY with ab170105 at a 1/100 dilution.
    • Immunofluorescence analysis of HepG2 cells labeling RALY with ab170105 at a 1/250 dilution.

    References

    ab170105 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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