Overview

  • Product name
    Anti-RAMP1 antibody [EPR10867]
    See all RAMP1 primary antibodies
  • Description
    Rabbit monoclonal [EPR10867] to RAMP1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human RAMP1. The exact sequence is proprietary.
    Database link: O60894

  • Positive control
    • WB: Human uterus, fetal brain, mouse brain, rat brain, human uterus and Caco-2 lysates.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab156575 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Function
      Transports the calcitonin gene-related peptide type 1 receptor (CALCRL) to the plasma membrane. Acts as a receptor for calcitonin-gene-related peptide (CGRP) together with CALCRL.
    • Tissue specificity
      Expressed in many tissues including the uterus, bladder, brain, pancreas and gastro-intestinal tract.
    • Sequence similarities
      Belongs to the RAMP family.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Calcitonin receptor like receptor activity modifying protein 1 antibody
      • Calcitonin-receptor-like receptor activity-modifying protein 1 antibody
      • CRLR activity modifying protein 1 antibody
      • CRLR activity-modifying protein 1 antibody
      • Ramp1 antibody
      • RAMP1_HUMAN antibody
      • receptor (calcitonin) activity modifying protein 1 antibody
      • Receptor (G protein coupled) activity modifying protein 1 antibody
      • Receptor activity modifying protein 1 [Precursor] antibody
      • Receptor activity-modifying protein 1 antibody
      see all

    Images

    • Anti-RAMP1 antibody [EPR10867] (ab156575) at 1/1000 dilution (purified) + Human uterus lysates at 15 µg

      Secondary
      Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 17 kDa



      Blocking and diluting buffer: 5% NFDM/TBST.

    • All lanes : Anti-RAMP1 antibody [EPR10867] (ab156575) at 1/5000 dilution (purified)

      Lane 1 : Mouse brain lysates
      Lane 2 : Rat brain lysates

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 17 kDa



      Blocking and diluting buffer: 5% NFDM/TBST.

    • All lanes : Anti-RAMP1 antibody [EPR10867] (ab156575) at 1/1000 dilution (unpurified)

      Lane 1 : Human uterus lysate
      Lane 2 : Human fetal brain lysate
      Lane 3 : Caco-2 (Human colorectal adenocarcinoma cell line) lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 17 kDa

    References

    This product has been referenced in:
    • Aubdool AA  et al. A Novel a-Calcitonin Gene-Related Peptide Analogue Protects Against End-Organ Damage in Experimental Hypertension, Cardiac Hypertrophy, and Heart Failure. Circulation 136:367-383 (2017). Read more (PubMed: 28446517) »
    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab156575.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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