Key features and details
- Rabbit polyclonal to RAMP3
- Suitable for: ICC/IF, WB, ELISA
- Reacts with: Recombinant fragment
- Isotype: IgG
Product nameAnti-RAMP3 antibody
See all RAMP3 primary antibodies
DescriptionRabbit polyclonal to RAMP3
Tested applicationsSuitable for: ICC/IF, WB, ELISAmore details
Species reactivityReacts with: Recombinant fragment
Predicted to work with: Human
A synthetic peptide corresponding to N-terminal residues of human RAMP3
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab58660 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 16 kDa. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.|
|ELISA||Use at an assay dependent dilution.|
RelevanceRAMP3 (receptor calcitonin activity modifying protein 3) transports the calcitonin gene-related peptide type 1 receptor (CALCRL) to the plasma membrane. It acts as a receptor for adrenomedullin (AM) together with CALCRL. It is strongly expressed in lung, breast, immune system and fetal tissues and belongs to the RAMP family.
Cellular localizationCell Membrane; Single-pass type I membrane protein
- Calcitonin receptor like receptor activity modifying protein 3 antibody
- CRLR activity modifying protein 3 antibody
- CRLR activity-modifying protein 3 antibody
ICC/IF image of ab58660 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab58660, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab58660 has been referenced in 1 publication.
- Brekhman V et al. Receptor activity modifying protein-3 mediates the protumorigenic activity of lysyl oxidase-like protein-2. FASEB J : (2010). PubMed: 20802105