Overview

  • Product name

    Anti-Ramp4-2 antibody [EPR14338]
    See all Ramp4-2 primary antibodies
  • Description

    Rabbit monoclonal [EPR14338] to Ramp4-2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human Ramp4-2 aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: Q8N6R1

  • Positive control

    • 293T, LnCaP and SW480 cell lysates; Human liver tissue; SW480 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 40% Glycerol, 59% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR14338
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab184571 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 7 kDa (predicted molecular weight: 7 kDa).
IHC-P 1/500.
ICC/IF 1/250.

Target

  • Function

    May interact with target proteins during translocation into the lumen of the endoplasmic reticulum. May protect unfolded target proteins against degradation and facilitate correct glycosylation.
  • Sequence similarities

    Belongs to the RAMP4 family.
  • Cellular localization

    Membrane. Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Ribosome-associated membrane protein RAMP4-2 antibody
    • SERP2 antibody
    • SERP2_HUMAN antibody
    • Stress-associated endoplasmic reticulum protein 2 antibody

Images

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed SW480 cells labeling Ramp4-2 with ab184571 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 diution. Counter stained with Dapi (blue).

  • Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Ramp4-2 with ab184571 at 1/500 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

  • All lanes : Anti-Ramp4-2 antibody [EPR14338] (ab184571) at 1/100000 dilution

    Lane 1 : LnCaP cell lysate
    Lane 2 : SW480 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 7 kDa
    Observed band size: 7 kDa

  • Anti-Ramp4-2 antibody [EPR14338] (ab184571) at 1/50000 dilution + 293T cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 7 kDa
    Observed band size: 7 kDa

References

ab184571 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab184571.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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