Overview

  • Product name

    Ran Activation Assay Kit
  • Sample type

    Tissue, Adherent cells, Suspension cells
  • Species reactivity

    Reacts with: Mouse, Rat, Chicken, Dog, Human
  • Product overview

    Ran Activation Assay Kit (ab211172) utilizes RanBP1 Agarose beads to selectively isolate and pull-down the active form of Ran from purified samples or endogenous lysates. Subsequently, the precipitated GTP-Ran is detected by western blot analysis using an anti-Ran antibody, which reacts with the human, mouse, rat, dog and chicken protein.


    Features: 1) non radioactive assay format; 2) fast results: 1 hour assay plus electrophoresis/blotting time; 3) includes Cdc42 positive control; 4) pink colored agarose beads for easy identification during washing and aspiration steps.

  • Notes

    Small GTP-binding proteins (or GTPases) are a family of proteins that serve as molecular regulators in signaling transduction pathways.

    Ran (Ras-related nuclear protein), a 26 kDa protein of the Ras superfamily, is involved in a variety of biological response pathways that include chromatin condensation, cell cycle progression, and translocation of RNA/proteins through the nuclear pore complex. Ran acts a negative regulator of the kinase activity of CRK1 and VRK2. Like other small GTPases, Ran regulates molecular events by cycling between an inactive GDP-bound form and an active GTP-bound form. In its active (GTP-bound) state, Ran binds specifically to RanBP1 to control downstream signaling cascades.

Properties

Associated products

Images

  • Ran positive control (10 µL of 293 cell lysate at 0.5 mg/mL) detected by immunoblot.

  • Ran activation assay. Lane 1. NIH 3T3 cell lysate loaded with GDP and incubated with RanBP1 Agarose beads. Lane 2. NIH 3T3 cell lysate loaded with GTPγS and incubated with RanBP1 Agarose beads.

Protocols

References

ab211172 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab211172.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up