Overview

  • Product name

    Anti-Ran antibody [8D1H12]
    See all Ran primary antibodies
  • Description

    Mouse monoclonal [8D1H12] to Ran
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Rat, Human, Monkey
    Predicted to work with: Mouse, Chicken, Cow, Dog, Cynomolgus monkey, Orangutan
  • Immunogen

    Recombinant full length protein corresponding to Human Ran aa 1-216. (Expressed in E.coli).
    Sequence:

    MAAQGEPQVQFKLVLVGDGGTGKTTFVKRHLTGEFEKKYVATLGVEVHPL VFHTNRGPIKFNVWDTAGQEKFGGLRDGYYIQAQCAIIMFDVTSRVTYKN VPNWHRDLVRVCENIPIVLCGNKVDIKDRKVKAKSIVFHRKKNLQYYDIS AKSNYNFEKPFLWLARKLIGDPNLEFVAMPALAPPEVVMDPALAAQYEHD LEVAQTTALPDEDDDL


    Database link: P62826

  • Positive control

    • WB: A431, C6, Jurkat, HeLa and COS-7 cell lysate; Recombinant human Ran protein; RAN (AA: 1-216)-hIgGFc transfected HEK-293 cell lysate. IHC-P: Human esophageal cancer and rectum cancer tissue. Flow: HeLa cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    8D1H12
  • Isotype

    IgG1

Applications

Our Abpromise guarantee covers the use of ab233762 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000.
IHC-P 1/200 - 1/1000.
Flow Cyt 1/200 - 1/400.

Target

  • Function

    GTP-binding protein involved in nucleocytoplasmic transport. Required for the import of protein into the nucleus and also for RNA export. Involved in chromatin condensation and control of cell cycle (By similarity). The complex with BIRC5/ survivin plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. Acts as a negative regulator of the kinase activity of VRK1 and VRK2.
    Enhances AR-mediated transactivation. Transactivation decreases as the poly-Gln length within AR increases.
  • Tissue specificity

    Expressed in a variety of tissues.
  • Sequence similarities

    Belongs to the small GTPase superfamily. Ran family.
  • Cellular localization

    Nucleus. Cytoplasm. Melanosome. Predominantly nuclear during interphase (By similarity). Becomes dispersed throughout the cytoplasm during mitosis. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links

  • Alternative names

    • Androgen receptor associated protein 24 antibody
    • Androgen receptor-associated protein 24 antibody
    • ARA24 antibody
    • Gsp1 antibody
    • GTP-binding nuclear protein Ran antibody
    • GTPase Ran antibody
    • Guanosine triphosphatase Ran antibody
    • Member RAS oncogene family antibody
    • OK/SW-cl.81 antibody
    • ran antibody
    • RAN member RAS oncogene family antibody
    • RAN_HUMAN antibody
    • RanGTPase antibody
    • Ras-like protein TC4 antibody
    • Ras-related nuclear protein antibody
    • TC4 antibody
    see all

Images

  • All lanes : Anti-Ran antibody [8D1H12] (ab233762) at 1/500 dilution

    Lane 1 : A431 (human epidermoid carcinoma cell line) cell lysate
    Lane 2 : C6 (Rat glial tumor cell line) cell lysate
    Lanes 3 & 6 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
    Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
    Lane 5 : COS-7 (African green monkey kidney fibroblast-like cell line) cell lysate
  • Paraffin-embedded human rectum cancer tissue stained for Ran using ab233762 at 1/200 dilution in immunohistochemical analysis. DAB staining.

  • Paraffin-embedded human esophageal cancer tissue stained for Ran using ab233762 at 1/200 dilution in immunohistochemical analysis. DAB staining.

  • Flow cytometric analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Ran using ab233762 at 1/200 dilution (green) compared to a negative control (red).

  • All lanes : Anti-Ran antibody [8D1H12] (ab233762) at 1/500 dilution

    Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
    Lane 2 : RAN (AA: 1-216)-hIgGFc transfected HEK-293 cell lysate
  • Anti-Ran antibody [8D1H12] (ab233762) at 1/500 dilution + Recombinant human Ran protein


    Expected MW is 50.4 kDa.

References

ab233762 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab233762.
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