Key features and details
- Rabbit polyclonal to Ran
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Ran antibody
See all Ran primary antibodies
DescriptionRabbit polyclonal to Ran
Tested applicationsSuitable for: IHC-P, WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Cow, Dog, Xenopus laevis, Zebrafish, Orangutan, Xenopus tropicalis
Synthetic peptide within Human Ran aa 166-216. The exact sequence is proprietary. (NP_006316.1).
Database link: P62826
- IHC-P: Human breast carcinoma tissue; Mouse renal cell carcinoma tissue. WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysates. IP: HEK-293T whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab226195 was affinity purified using an epitope specific to Ran immobilized on solid support.
Our Abpromise guarantee covers the use of ab226195 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/2000 - 1/10000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 24 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionGTP-binding protein involved in nucleocytoplasmic transport. Required for the import of protein into the nucleus and also for RNA export. Involved in chromatin condensation and control of cell cycle (By similarity). The complex with BIRC5/ survivin plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. Acts as a negative regulator of the kinase activity of VRK1 and VRK2.
Enhances AR-mediated transactivation. Transactivation decreases as the poly-Gln length within AR increases.
Tissue specificityExpressed in a variety of tissues.
Sequence similaritiesBelongs to the small GTPase superfamily. Ran family.
Cellular localizationNucleus. Cytoplasm. Melanosome. Predominantly nuclear during interphase (By similarity). Becomes dispersed throughout the cytoplasm during mitosis. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- Androgen receptor associated protein 24 antibody
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All lanes : Anti-Ran antibody (ab226195) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 24 kDa
Exposure time: 30 seconds
Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for Ran using ab226195 at 1/5000 dilution in immunohistochemical analysis. Detection: DAB staining.
Ran was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226195 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab226195 at 1 µg/ml.
Lane 1: ab226195 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
Formalin-fixed, paraffin-embedded mouse renal cell carcinoma tissue stained for Ran using ab226195 at 1/5000 dilution in immunohistochemical analysis. Detection: DAB staining.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab226195 has not yet been referenced specifically in any publications.