Overview

  • Product name

  • Description

    Rabbit polyclonal to Ran
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken, Cow, Dog, Xenopus laevis, Zebrafish, Orangutan, Xenopus tropicalis
  • Immunogen

    Synthetic peptide within Human Ran aa 166-216. The exact sequence is proprietary. (NP_006316.1).
    Database link: P62826

  • Positive control

    • IHC-P: Human breast carcinoma tissue; Mouse renal cell carcinoma tissue. WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysates. IP: HEK-293T whole cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab226195 was affinity purified using an epitope specific to Ran immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab226195 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/2000 - 1/10000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Predicted molecular weight: 24 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    GTP-binding protein involved in nucleocytoplasmic transport. Required for the import of protein into the nucleus and also for RNA export. Involved in chromatin condensation and control of cell cycle (By similarity). The complex with BIRC5/ survivin plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. Acts as a negative regulator of the kinase activity of VRK1 and VRK2.
    Enhances AR-mediated transactivation. Transactivation decreases as the poly-Gln length within AR increases.
  • Tissue specificity

    Expressed in a variety of tissues.
  • Sequence similarities

    Belongs to the small GTPase superfamily. Ran family.
  • Cellular localization

    Nucleus. Cytoplasm. Melanosome. Predominantly nuclear during interphase (By similarity). Becomes dispersed throughout the cytoplasm during mitosis. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links

  • Alternative names

    • Androgen receptor associated protein 24 antibody
    • Androgen receptor-associated protein 24 antibody
    • ARA24 antibody
    • Gsp1 antibody
    • GTP-binding nuclear protein Ran antibody
    • GTPase Ran antibody
    • Guanosine triphosphatase Ran antibody
    • Member RAS oncogene family antibody
    • OK/SW-cl.81 antibody
    • ran antibody
    • RAN member RAS oncogene family antibody
    • RAN_HUMAN antibody
    • RanGTPase antibody
    • Ras-like protein TC4 antibody
    • Ras-related nuclear protein antibody
    • TC4 antibody
    see all

Images

  • All lanes : Anti-Ran antibody (ab226195) at 0.1 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
    Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 24 kDa


    Exposure time: 30 seconds
  • Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for Ran using ab226195 at 1/5000 dilution in immunohistochemical analysis. Detection: DAB staining.

  • Ran was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226195 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab226195 at 1 µg/ml.

    Lane 1: ab226195 IP in HEK-293T whole cell lysate.

    Lane 2: Control IgG IP in HEK-293T whole cell lysate.

    Detection: Chemiluminescence with exposure time of 10 seconds.

  • Formalin-fixed, paraffin-embedded mouse renal cell carcinoma tissue stained for Ran using ab226195 at 1/5000 dilution in immunohistochemical analysis. Detection: DAB staining.

References

ab226195 has not yet been referenced specifically in any publications.

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