Product nameAnti-Ran antibody
See all Ran primary antibodies
DescriptionRabbit polyclonal to Ran
SpecificityThis antibody detects endogenous levels of total Ran protein.
Tested applicationsSuitable for: ICC/IF, WB, IHC-P, ELISAmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide derived from Human Ran
- Human breast carcinoma tissue and extracts from LOVO cells
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Concentration information loading...
PurityImmunogen affinity purified
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
Our Abpromise guarantee covers the use of ab53775 in the following tested applications.
|ICC/IF||Use at an assay dependent concentration. PubMed: 23308199|
|WB||1/500 - 1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).|
|IHC-P||Use at an assay dependent concentration.|
FunctionGTP-binding protein involved in nucleocytoplasmic transport. Required for the import of protein into the nucleus and also for RNA export. Involved in chromatin condensation and control of cell cycle (By similarity). The complex with BIRC5/ survivin plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules.
Enhances AR-mediated transactivation. Transactivation decreases as the poly-Gln length within AR increases.
Tissue specificityExpressed in a variety of tissues.
Sequence similaritiesBelongs to the small GTPase superfamily. Ran family.
modificationsThe N-terminus is blocked.
Cellular localizationNucleus. Cytoplasm. Melanosome. Becomes dispersed throughout the cytoplasm during mitosis. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
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All lanes : Anti-Ran antibody (ab53775) at 1/500 dilution
Lane 1 : Extracts from LOVO cells
Lane 2 : Extracts from LOVO cells, plus immunising peptide
Predicted band size: 26 kDa
Observed band size: 26 kDa
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab53775 at a 1/50 dilution.
Left image un-treated.
Right image treated with immunizing peptide.
This product has been referenced in:
- Connell M et al. HMMR acts in the PLK1-dependent spindle positioning pathway and supports neural development. Elife 6:N/A (2017). Read more (PubMed: 28994651) »
- Khosravi B et al. Cytoplasmic poly-GA aggregates impair nuclear import of TDP-43 in C9orf72 ALS/FTLD. Hum Mol Genet 26:790-800 (2017). Read more (PubMed: 28040728) »