- Datasheet
- Specific References (5)
- Protocols
Overview
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Product nameAnti-Ran antibody
See all Ran primary antibodies -
DescriptionRabbit polyclonal to Ran
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Host speciesRabbit
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SpecificityThis antibody detects endogenous levels of total Ran protein.
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Tested applicationsSuitable for: ICC/IF, WB, IHC-P, ELISAmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide derived from Human Ran
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Positive control
- Human breast carcinoma tissue and extracts from LOVO cells
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Assay kits
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab53775 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. PubMed: 23308199 | |
| WB | 1/500 - 1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa). | |
| IHC-P | Use at an assay dependent concentration. | |
| ELISA | 1/10000. |
Target
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FunctionGTP-binding protein involved in nucleocytoplasmic transport. Required for the import of protein into the nucleus and also for RNA export. Involved in chromatin condensation and control of cell cycle (By similarity). The complex with BIRC5/ survivin plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules.
Enhances AR-mediated transactivation. Transactivation decreases as the poly-Gln length within AR increases. -
Tissue specificityExpressed in a variety of tissues.
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Sequence similaritiesBelongs to the small GTPase superfamily. Ran family.
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Post-translational
modificationsThe N-terminus is blocked. -
Cellular localizationNucleus. Cytoplasm. Melanosome. Becomes dispersed throughout the cytoplasm during mitosis. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
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Database links
- Entrez Gene: 5901 Human
- Entrez Gene: 100045999 Mouse
- Entrez Gene: 19384 Mouse
- Entrez Gene: 84509 Rat
- Omim: 601179 Human
- SwissProt: P62826 Human
- SwissProt: P62827 Mouse
- SwissProt: P62828 Rat
see all -
Alternative names
- Androgen receptor associated protein 24 antibody
- Androgen receptor-associated protein 24 antibody
- ARA 24 antibody
see all
Images
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Western blot - Anti-Ran antibody (ab53775)All lanes : Anti-Ran antibody (ab53775) at 1/500 dilution
Lane 1 : Extracts from LOVO cells
Lane 2 : Extracts from LOVO cells, plus immunising peptide
Predicted band size: 26 kDa
Observed band size: 26 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ran antibody (ab53775)Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab53775 at a 1/50 dilution.Left image un-treated.
Right image treated with immunizing peptide.
Protocols
References
This product has been referenced in:
- Connell M et al. HMMR acts in the PLK1-dependent spindle positioning pathway and supports neural development. Elife 6:N/A (2017). Read more (PubMed: 28994651) »
- Mastroeni D et al. Reduced RAN expression and disrupted transport between cytoplasm and nucleus; a key event in Alzheimer's disease pathophysiology. PLoS One 8:e53349 (2013). WB, IHC-P, ICC/IF ; Human . Read more (PubMed: 23308199) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"