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    ran-antibody-ab53775.pdf

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Cell Biology Cell Cycle Cell Division Spindle
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Anti-Ran antibody (ab53775)

  • Datasheet
Reviews (2) Submit a question References (8)

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Western blot - Anti-Ran antibody (ab53775)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ran antibody (ab53775)

Key features and details

  • Rabbit polyclonal to Ran
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Protein
Recombinant Human Ran (mutated T24N) protein (ab90206)

View more associated products

Overview

  • Product name

    Anti-Ran antibody
    See all Ran primary antibodies
  • Description

    Rabbit polyclonal to Ran
  • Host species

    Rabbit
  • Specificity

    This antibody detects endogenous levels of total Ran protein.
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide derived from Human Ran

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Spindle
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • Small G Proteins
    • Ras Family
    • Signal Transduction
    • Protein Trafficking
    • Nuclear Import / Export
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Co-activators/co-repressors
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Nuclear Pore Complex

Associated products

  • Assay kits

    • Ran Activation Assay Kit (ab211172)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Ran (mutated T24N) protein (ab90206)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab53775 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Human
Application Abreviews Notes
WB (1)
1/500 - 1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
IHC-P
Use at an assay dependent concentration.
Notes
WB
1/500 - 1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
IHC-P
Use at an assay dependent concentration.

Target

  • Function

    GTP-binding protein involved in nucleocytoplasmic transport. Required for the import of protein into the nucleus and also for RNA export. Involved in chromatin condensation and control of cell cycle (By similarity). The complex with BIRC5/ survivin plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules.
    Enhances AR-mediated transactivation. Transactivation decreases as the poly-Gln length within AR increases.
  • Tissue specificity

    Expressed in a variety of tissues.
  • Sequence similarities

    Belongs to the small GTPase superfamily. Ran family.
  • Post-translational
    modifications

    The N-terminus is blocked.
  • Cellular localization

    Nucleus. Cytoplasm. Melanosome. Becomes dispersed throughout the cytoplasm during mitosis. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Target information above from: UniProt accession P62826 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5901 Human
    • Omim: 601179 Human
    • SwissProt: P62826 Human
    • Unigene: 10842 Human
    • Alternative names

      • Androgen receptor associated protein 24 antibody
      • Androgen receptor-associated protein 24 antibody
      • ARA 24 antibody
      • ARA24 antibody
      • Gsp1 antibody
      • GTP binding nuclear protein RAN antibody
      • GTP-binding nuclear protein Ran antibody
      • GTPase Ran antibody
      • Guanosine triphosphatase Ran antibody
      • LPS antibody
      • OK/SW-cl.81 antibody
      • ran antibody
      • RAN member RAS oncogene family antibody
      • RAN_HUMAN antibody
      • RanGTPase antibody
      • Ras like protein TC4 antibody
      • Ras related nuclear protein antibody
      • Ras-like protein TC4 antibody
      • Ras-related nuclear protein antibody
      • RASL2 8 antibody
      • TC 4 antibody
      • TC4 antibody
      see all

    Images

    • Western blot - Anti-Ran antibody (ab53775)
      Western blot - Anti-Ran antibody (ab53775)
      All lanes : Anti-Ran antibody (ab53775) at 1/500 dilution

      Lane 1 : Extracts from LOVO cells
      Lane 2 : Extracts from LOVO cells, plus immunising peptide

      Predicted band size: 26 kDa
      Observed band size: 26 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ran antibody (ab53775)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ran antibody (ab53775)
      Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab53775 at a 1/50 dilution.

      Left image un-treated.

      Right image treated with immunizing peptide.

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (8)

    Publishing research using ab53775? Please let us know so that we can cite the reference in this datasheet.

    ab53775 has been referenced in 8 publications.

    • Feng H  et al. MiR-802 Suppresses Colorectal Cancer Cell Viability, Migration and Invasion by Targeting RAN. Cancer Manag Res 12:2291-2300 (2020). PubMed: 32273766
    • Zhong Y  et al. Lin28A Regulates Stem-like Properties of Ovarian Cancer Cells by Enriching RAN and HSBP1 mRNA and Up-regulating its Protein Expression. Int J Biol Sci 16:1941-1953 (2020). PubMed: 32398961
    • Connell M  et al. HMMR acts in the PLK1-dependent spindle positioning pathway and supports neural development. Elife 6:N/A (2017). PubMed: 28994651
    • Khosravi B  et al. Cytoplasmic poly-GA aggregates impair nuclear import of TDP-43 in C9orf72 ALS/FTLD. Hum Mol Genet 26:790-800 (2017). PubMed: 28040728
    • Mastroeni D  et al. Reduced RAN expression and disrupted transport between cytoplasm and nucleus; a key event in Alzheimer's disease pathophysiology. PLoS One 8:e53349 (2013). WB, IHC-P, ICC/IF ; Human . PubMed: 23308199
    • Lecompte S  et al. Genetic and molecular insights into the role of PROX1 in glucose metabolism. Diabetes 62:1738-45 (2013). PubMed: 23274905
    • Amengual J  et al. A mitochondrial enzyme degrades carotenoids and protects against oxidative stress. FASEB J 25:948-59 (2011). WB ; Mouse . PubMed: 21106934
    • Lobo GP  et al. {beta},{beta}-Carotene Decreases Peroxisome Proliferator Receptor {gamma} Activity and Reduces Lipid Storage Capacity of Adipocytes in a {beta},{beta}-Carotene Oxygenase 1-dependent Manner. J Biol Chem 285:27891-9 (2010). WB ; Mouse . PubMed: 20573961

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Ran antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (SAOS)
    Permeabilization
    Yes - NP40
    Specification
    SAOS
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 07 2018

    Western blot abreview for Anti-Ran antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    15 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Human Cell lysate - whole cell (U2OS)
    Specification
    U2OS
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 09 2014

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