Anti-Ran antibody (ab53775)
Key features and details
- Rabbit polyclonal to Ran
- Suitable for: ELISA, ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Ran antibody
See all Ran primary antibodies -
Description
Rabbit polyclonal to Ran -
Host species
Rabbit -
Tested applications
Suitable for: ELISA, ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide derived from Human Ran
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab53775 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use at an assay dependent concentration.
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ICC/IF | (1) |
Use at an assay dependent concentration. PubMed: 23308199
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WB | (1) |
1/500 - 1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
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IHC-P |
Use at an assay dependent concentration.
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Notes |
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ELISA
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. PubMed: 23308199 |
WB
1/500 - 1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa). |
IHC-P
Use at an assay dependent concentration. |
Target
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Function
GTP-binding protein involved in nucleocytoplasmic transport. Required for the import of protein into the nucleus and also for RNA export. Involved in chromatin condensation and control of cell cycle (By similarity). The complex with BIRC5/ survivin plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules.
Enhances AR-mediated transactivation. Transactivation decreases as the poly-Gln length within AR increases. -
Tissue specificity
Expressed in a variety of tissues. -
Sequence similarities
Belongs to the small GTPase superfamily. Ran family. -
Post-translational
modificationsThe N-terminus is blocked. -
Cellular localization
Nucleus. Cytoplasm. Melanosome. Becomes dispersed throughout the cytoplasm during mitosis. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 5901 Human
- Entrez Gene: 100045999 Mouse
- Entrez Gene: 19384 Mouse
- Entrez Gene: 84509 Rat
- Omim: 601179 Human
- SwissProt: P62826 Human
- SwissProt: P62827 Mouse
- SwissProt: P62828 Rat
see all -
Alternative names
- Androgen receptor associated protein 24 antibody
- Androgen receptor-associated protein 24 antibody
- ARA 24 antibody
see all
Images
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All lanes : Anti-Ran antibody (ab53775) at 1/500 dilution
Lane 1 : Extracts from LOVO cells
Lane 2 : Extracts from LOVO cells, plus immunising peptide
Predicted band size: 26 kDa
Observed band size: 26 kDa -
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab53775 at a 1/50 dilution.
Left image un-treated.
Right image treated with immunizing peptide.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab53775 has been referenced in 9 publications.
- Yoo TY & Mitchison TJ O-GlcNAc modification of nuclear pore complexes accelerates bidirectional transport. J Cell Biol 220:N/A (2021). PubMed: 33909044
- Feng H et al. MiR-802 Suppresses Colorectal Cancer Cell Viability, Migration and Invasion by Targeting RAN. Cancer Manag Res 12:2291-2300 (2020). PubMed: 32273766
- Zhong Y et al. Lin28A Regulates Stem-like Properties of Ovarian Cancer Cells by Enriching RAN and HSBP1 mRNA and Up-regulating its Protein Expression. Int J Biol Sci 16:1941-1953 (2020). PubMed: 32398961
- Connell M et al. HMMR acts in the PLK1-dependent spindle positioning pathway and supports neural development. Elife 6:N/A (2017). PubMed: 28994651
- Khosravi B et al. Cytoplasmic poly-GA aggregates impair nuclear import of TDP-43 in C9orf72 ALS/FTLD. Hum Mol Genet 26:790-800 (2017). PubMed: 28040728
- Mastroeni D et al. Reduced RAN expression and disrupted transport between cytoplasm and nucleus; a key event in Alzheimer's disease pathophysiology. PLoS One 8:e53349 (2013). WB, IHC-P, ICC/IF ; Human . PubMed: 23308199
- Lecompte S et al. Genetic and molecular insights into the role of PROX1 in glucose metabolism. Diabetes 62:1738-45 (2013). PubMed: 23274905
- Amengual J et al. A mitochondrial enzyme degrades carotenoids and protects against oxidative stress. FASEB J 25:948-59 (2011). WB ; Mouse . PubMed: 21106934
- Lobo GP et al. {beta},{beta}-Carotene Decreases Peroxisome Proliferator Receptor {gamma} Activity and Reduces Lipid Storage Capacity of Adipocytes in a {beta},{beta}-Carotene Oxygenase 1-dependent Manner. J Biol Chem 285:27891-9 (2010). WB ; Mouse . PubMed: 20573961