Product nameAnti-Ran antibody - C-terminal
See all Ran primary antibodies
DescriptionRabbit polyclonal to Ran - C-terminal
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Cow, Dog, Xenopus laevis, Orangutan
Synthetic peptide within Human Ran aa 175-216 (C terminal). The exact sequence is proprietary. NP_006316.1
Database link: P62826
- IHC-P: Human breast carcinoma and mouse renal cell carcinoma tissue. IP: HEK-293T whole cell lysate. WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab226205 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 24 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
|IHC-P||1/2000 - 1/10000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionGTP-binding protein involved in nucleocytoplasmic transport. Required for the import of protein into the nucleus and also for RNA export. Involved in chromatin condensation and control of cell cycle (By similarity). The complex with BIRC5/ survivin plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. Acts as a negative regulator of the kinase activity of VRK1 and VRK2.
Enhances AR-mediated transactivation. Transactivation decreases as the poly-Gln length within AR increases.
Tissue specificityExpressed in a variety of tissues.
Sequence similaritiesBelongs to the small GTPase superfamily. Ran family.
Cellular localizationNucleus. Cytoplasm. Melanosome. Predominantly nuclear during interphase (By similarity). Becomes dispersed throughout the cytoplasm during mitosis. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- Androgen receptor associated protein 24 antibody
- Androgen receptor-associated protein 24 antibody
- ARA24 antibody
All lanes : Anti-Ran antibody - C-terminal (ab226205) at 0.1 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 24 kDa
Exposure time: 3 minutes
Formalin fixed, paraffin embedded human breast carcinoma tissue stained for Ran with ab226205 (1/5000 dilution) in immunohistochemical analysis. DAB detection.
Ran was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226205 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226205 at 1 µg/ml.
Lane 1: ab226205 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
Formalin fixed, paraffin embedded mouse renal cell carcinoma tissue stained for Ran with ab226205 (1/5000 dilution) in immunohistochemical analysis. DAB detection.
ab226205 has not yet been referenced specifically in any publications.