Overview

  • Product name

    Anti-Ran antibody [EPR10790(B)]
    See all Ran primary antibodies
  • Description

    Rabbit monoclonal [EPR10790(B)] to Ran
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to amino acid residues in Human Ran was used as an immunogen (UniProt ID P62826).

  • Positive control

    • 293T, HeLa and Jurkat whole cell lysate (ab7899). 293T cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab157213 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 24 kDa.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function

      GTP-binding protein involved in nucleocytoplasmic transport. Required for the import of protein into the nucleus and also for RNA export. Involved in chromatin condensation and control of cell cycle (By similarity). The complex with BIRC5/ survivin plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules.
      Enhances AR-mediated transactivation. Transactivation decreases as the poly-Gln length within AR increases.
    • Tissue specificity

      Expressed in a variety of tissues.
    • Sequence similarities

      Belongs to the small GTPase superfamily. Ran family.
    • Post-translational
      modifications

      The N-terminus is blocked.
    • Cellular localization

      Nucleus. Cytoplasm. Melanosome. Becomes dispersed throughout the cytoplasm during mitosis. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt
    • Database links

    • Alternative names

      • Androgen receptor associated protein 24 antibody
      • Androgen receptor-associated protein 24 antibody
      • ARA 24 antibody
      • ARA24 antibody
      • Gsp1 antibody
      • GTP binding nuclear protein RAN antibody
      • GTP-binding nuclear protein Ran antibody
      • GTPase Ran antibody
      • Guanosine triphosphatase Ran antibody
      • LPS antibody
      • OK/SW-cl.81 antibody
      • ran antibody
      • RAN member RAS oncogene family antibody
      • RAN_HUMAN antibody
      • RanGTPase antibody
      • Ras like protein TC4 antibody
      • Ras related nuclear protein antibody
      • Ras-like protein TC4 antibody
      • Ras-related nuclear protein antibody
      • RASL2 8 antibody
      • TC 4 antibody
      • TC4 antibody
      see all

    Images

    • All lanes : Anti-Ran antibody [EPR10790(B)] (ab157213) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 24 kDa

    • Immunofluorescent analysis of 293T cells, labeling Ran with ab157213 at 1/100 dilution.

    References

    This product has been referenced in:

    • Deng Q  et al. HepaCAM inhibits cell proliferation and invasion in prostate cancer by suppressing nuclear translocation of the androgen receptor via its cytoplasmic domain. Mol Med Rep 19:2115-2124 (2019). Read more (PubMed: 30664187) »
    • Wang F  et al. Nuclear translocation of fibroblast growth factor-2 (FGF2) is regulated by Karyopherin-ß2 and Ran GTPase in human glioblastoma cells. Oncotarget 6:21468-78 (2015). Read more (PubMed: 26056081) »
    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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