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RecombinantRabMAb

Recombinant Anti-Ran antibody [EPR10790(B)] - BSA and Azide free (ab249315)

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  • Certificate of Compliance
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Western blot - Anti-Ran antibody [EPR10790(B)] - BSA and Azide free (ab249315)
  • Immunocytochemistry - Anti-Ran antibody [EPR10790(B)] - BSA and Azide free (ab249315)
  • Anti-Ran antibody [EPR10790(B)] - BSA and Azide free (ab249315)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10790(B)] to Ran - BSA and Azide free
  • Suitable for: ICC, WB
  • Reacts with: Rat, Human

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Conjugation
Product image
Alexa Fluor® 488 Conjugation Kit (Fast) - Lightning-Link® (ab236553)
Protein
Recombinant Human Ran (mutated T24N) protein (ab90206)

View more associated products

Overview

  • Product name

    Anti-Ran antibody [EPR10790(B)] - BSA and Azide free
    See all Ran primary antibodies
  • Description

    Rabbit monoclonal [EPR10790(B)] to Ran - BSA and Azide free
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab249315 is the carrier-free version of ab157213. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab249315 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR10790(B)
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Spindle
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • Small G Proteins
    • Ras Family
    • Signal Transduction
    • Protein Trafficking
    • Nuclear Import / Export
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Co-activators/co-repressors
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Nuclear Pore Complex

Associated products

  • Alternative Versions

    • Anti-Ran antibody [EPR10790(B)] (ab157213)
  • Assay kits

    • Ran Activation Assay Kit (ab211172)
  • Conjugation kits

    • FITC Conjugation Kit (Fast) - Lightning-Link® (ab188285)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)
    • Jurkat whole cell lysate (ab7899)
  • Recombinant Protein

    • Recombinant Human Ran (mutated T24N) protein (ab90206)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab249315 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
All applications
Mouse
Application Abreviews Notes
ICC
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 24 kDa.
Notes
ICC
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 24 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function

      GTP-binding protein involved in nucleocytoplasmic transport. Required for the import of protein into the nucleus and also for RNA export. Involved in chromatin condensation and control of cell cycle (By similarity). The complex with BIRC5/ survivin plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules.
      Enhances AR-mediated transactivation. Transactivation decreases as the poly-Gln length within AR increases.
    • Tissue specificity

      Expressed in a variety of tissues.
    • Sequence similarities

      Belongs to the small GTPase superfamily. Ran family.
    • Post-translational
      modifications

      The N-terminus is blocked.
    • Cellular localization

      Nucleus. Cytoplasm. Melanosome. Becomes dispersed throughout the cytoplasm during mitosis. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Target information above from: UniProt accession P62826 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 5901 Human
      • Entrez Gene: 100045999 Mouse
      • Entrez Gene: 19384 Mouse
      • Entrez Gene: 84509 Rat
      • Omim: 601179 Human
      • SwissProt: P62826 Human
      • SwissProt: P62827 Mouse
      • SwissProt: P62828 Rat
      • Unigene: 10842 Human
      • Unigene: 297440 Mouse
      • Unigene: 386831 Mouse
      • Unigene: 107698 Rat
      see all
    • Alternative names

      • Androgen receptor associated protein 24 antibody
      • Androgen receptor-associated protein 24 antibody
      • ARA 24 antibody
      • ARA24 antibody
      • Gsp1 antibody
      • GTP binding nuclear protein RAN antibody
      • GTP-binding nuclear protein Ran antibody
      • GTPase Ran antibody
      • Guanosine triphosphatase Ran antibody
      • LPS antibody
      • OK/SW-cl.81 antibody
      • ran antibody
      • RAN member RAS oncogene family antibody
      • RAN_HUMAN antibody
      • RanGTPase antibody
      • Ras like protein TC4 antibody
      • Ras related nuclear protein antibody
      • Ras-like protein TC4 antibody
      • Ras-related nuclear protein antibody
      • RASL2 8 antibody
      • TC 4 antibody
      • TC4 antibody
      see all

    Images

    • Western blot - Anti-Ran antibody [EPR10790(B)] - BSA and Azide free (ab249315)
      Western blot - Anti-Ran antibody [EPR10790(B)] - BSA and Azide free (ab249315)
      All lanes : Anti-Ran antibody [EPR10790(B)] (ab157213) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 24 kDa



      This data was developed using ab157213, the same antibody clone in a different buffer formulation.

    • Immunocytochemistry - Anti-Ran antibody [EPR10790(B)] - BSA and Azide free (ab249315)
      Immunocytochemistry - Anti-Ran antibody [EPR10790(B)] - BSA and Azide free (ab249315)
      This data was developed using ab157213, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 293T cells, labeling Ran with ab157213 at 1/100 dilution.
    • Anti-Ran antibody [EPR10790(B)] - BSA and Azide free (ab249315)
      Anti-Ran antibody [EPR10790(B)] - BSA and Azide free (ab249315)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (0)

    Publishing research using ab249315? Please let us know so that we can cite the reference in this datasheet.

    ab249315 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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