RecombinantRabMAb

Recombinant Anti-Ran antibody [EPR10791(B)] (ab155103)

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Western blot - Anti-Ran antibody [EPR10791(B)] (ab155103)
  • Immunocytochemistry - Anti-Ran antibody [EPR10791(B)] (ab155103)
  • Flow Cytometry (Intracellular) - Anti-Ran antibody [EPR10791(B)] (ab155103)
  • Immunoprecipitation - Anti-Ran antibody [EPR10791(B)] (ab155103)
  • Anti-Ran antibody [EPR10791(B)] (ab155103)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10791(B)] to Ran
  • Suitable for: ICC, WB, IP, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-Ran antibody [EPR10791(B)]
    See all Ran primary antibodies

  • Description

    Rabbit monoclonal [EPR10791(B)] to Ran

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, WB, IP, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Ran. The exact sequence is proprietary.
    Database link: P62826
    (Peptide available as ab217263)

  • Positive control

    • WB: HepG2, LoVo, Jurkat, HeLa, C6, Raw 264.7, PC-12, and NIH/3T3 whole cell lysates; IP: HeLa whole cell lysate; Flow Cyt: HeLa cells; ICC: HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab155103 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC
1/100.

For unpurified use at 1/250 - 1/500.
WB
1/1000. Predicted molecular weight: 24 kDa.

For unpurified use at 1/1000 - 1/10000.
IP
1/50.

For unpurified use at 1/10 - 1/100.
Flow Cyt
1/100.

For unpurified use at 1/100 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
Notes
ICC
1/100.

For unpurified use at 1/250 - 1/500.
WB
1/1000. Predicted molecular weight: 24 kDa.

For unpurified use at 1/1000 - 1/10000.
IP
1/50.

For unpurified use at 1/10 - 1/100.
Flow Cyt
1/100.

For unpurified use at 1/100 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    GTP-binding protein involved in nucleocytoplasmic transport. Required for the import of protein into the nucleus and also for RNA export. Involved in chromatin condensation and control of cell cycle (By similarity). The complex with BIRC5/ survivin plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules.
    Enhances AR-mediated transactivation. Transactivation decreases as the poly-Gln length within AR increases.

  • Tissue specificity

    Expressed in a variety of tissues.

  • Sequence similarities

    Belongs to the small GTPase superfamily. Ran family.

  • Post-translational
    modifications

    The N-terminus is blocked.

  • Cellular localization

    Nucleus. Cytoplasm. Melanosome. Becomes dispersed throughout the cytoplasm during mitosis. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Androgen receptor associated protein 24 antibody
    • Androgen receptor-associated protein 24 antibody
    • ARA 24 antibody
    • ARA24 antibody
    • Gsp1 antibody
    • GTP binding nuclear protein RAN antibody
    • GTP-binding nuclear protein Ran antibody
    • GTPase Ran antibody
    • Guanosine triphosphatase Ran antibody
    • LPS antibody
    • OK/SW-cl.81 antibody
    • ran antibody
    • RAN member RAS oncogene family antibody
    • RAN_HUMAN antibody
    • RanGTPase antibody
    • Ras like protein TC4 antibody
    • Ras related nuclear protein antibody
    • Ras-like protein TC4 antibody
    • Ras-related nuclear protein antibody
    • RASL2 8 antibody
    • TC 4 antibody
    • TC4 antibody
    see all

Images

  • Western blot - Anti-Ran antibody [EPR10791(B)] (ab155103)
    Western blot - Anti-Ran antibody [EPR10791(B)] (ab155103)
    All lanes : Anti-Ran antibody [EPR10791(B)] (ab155103) at 1/1000 dilution (purified)

    Lane 1 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate
    Lane 2 : LoVo (Human colorectal adenocarcinoma epithelial cell) whole cell lysate
    Lane 3 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
    Lane 5 : C6 (Rat glial tumor glial cell) whole cell lysate
    Lane 6 : Raw 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
    Lane 7 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
    Lane 8 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 24 kDa
    Observed band size: 24 kDa



    We are unsure how to define the extra bands.

  • Immunocytochemistry - Anti-Ran antibody [EPR10791(B)] (ab155103)
    Immunocytochemistry - Anti-Ran antibody [EPR10791(B)] (ab155103)

    Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Ran with purified ab155103 at 1/100 dilution (10 µg/mL). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Flow Cytometry (Intracellular) - Anti-Ran antibody [EPR10791(B)] (ab155103)
    Flow Cytometry (Intracellular) - Anti-Ran antibody [EPR10791(B)] (ab155103)

    Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Ran with purified ab155103 at 1/100 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Immunoprecipitation - Anti-Ran antibody [EPR10791(B)] (ab155103)
    Immunoprecipitation - Anti-Ran antibody [EPR10791(B)] (ab155103)

    Purified ab155103 at 1/50 dilution (2 µg) immunoprecipitating Ran in HeLa whole cell lysate.
    Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10 µg
    Lane 2 (+): ab155103 + HeLa whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab155103 in HeLa whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 24 kDa

  • Anti-Ran antibody [EPR10791(B)] (ab155103)
    Anti-Ran antibody [EPR10791(B)] (ab155103)

References (1)

Publishing research using ab155103? Please let us know so that we can cite the reference in this datasheet.

ab155103 has been referenced in 1 publication.

  • Zhang H  et al. Mechanisms controlling the smooth muscle cell death in progeria via down-regulation of poly(ADP-ribose) polymerase 1. Proc Natl Acad Sci U S A 111:E2261-70 (2014). PubMed: 24843141

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