Recombinant
Recombinant Anti-Ran antibody [EPR10791(B)] (ab155103)
- Datasheet
- References (1)
- Protocols
Overview
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Product nameAnti-Ran antibody [EPR10791(B)]
See all Ran primary antibodies -
DescriptionRabbit monoclonal [EPR10791(B)] to Ran
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Host speciesRabbit
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Tested applicationsSuitable for: WB, ICC/IF, IP, Flow Cytmore details
Unsuitable for: IHC-P -
Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide, corresponding to residues in Human Ran (UniProt: P62826).
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Positive control
- HepG2, Lovo, Jurkat, HeLa, C6, RAW264.7, PC12 and NIH 3T3 cell lysates; HepG2 cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20ºC.
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Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR10791(B)
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Assay kits
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab155103 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 24 kDa.Can be blocked with Ran peptide (ab217263). | |
| ICC/IF | 1/250 - 1/500. | |
| IP | 1/10 - 1/100. | |
| Flow Cyt | 1/100 - 1/1000. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Application notes
Is unsuitable for IHC-P.
Target
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FunctionGTP-binding protein involved in nucleocytoplasmic transport. Required for the import of protein into the nucleus and also for RNA export. Involved in chromatin condensation and control of cell cycle (By similarity). The complex with BIRC5/ survivin plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules.
Enhances AR-mediated transactivation. Transactivation decreases as the poly-Gln length within AR increases. -
Tissue specificityExpressed in a variety of tissues.
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Sequence similaritiesBelongs to the small GTPase superfamily. Ran family.
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Post-translational
modificationsThe N-terminus is blocked. -
Cellular localizationNucleus. Cytoplasm. Melanosome. Becomes dispersed throughout the cytoplasm during mitosis. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
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Database links
- Entrez Gene: 5901 Human
- Entrez Gene: 100045999 Mouse
- Entrez Gene: 19384 Mouse
- Entrez Gene: 84509 Rat
- Omim: 601179 Human
- SwissProt: P62826 Human
- SwissProt: P62827 Mouse
- SwissProt: P62828 Rat
see all -
Alternative names
- Androgen receptor associated protein 24 antibody
- Androgen receptor-associated protein 24 antibody
- ARA 24 antibody
see all
Images
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![Western blot - Anti-Ran antibody [EPR10791(B)] (ab155103)](https://www.abcam.com/ps/products/155/ab155103/Images/Ran-Primary-antibodies-ab155103-1.jpg)
Western blot - Anti-Ran antibody [EPR10791(B)] (ab155103)All lanes : Anti-Ran antibody [EPR10791(B)] (ab155103) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : Lovo cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HeLa cell lysate
Lane 5 : C6 cell lysate
Lane 6 : RAW264.7 cell lysate
Lane 7 : PC12 cell lysate
Lane 8 : NIH 3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 24 kDa -
![Flow Cytometry - Anti-Ran antibody [EPR10791(B)] (ab155103)](https://www.abcam.com/ps/products/155/ab155103/Images/ab155103-3-ab155103FC.jpg)
Flow Cytometry - Anti-Ran antibody [EPR10791(B)] (ab155103)Overlay histogram showing HeLa cells stained with ab155103 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab155103, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions. -
![Immunocytochemistry/ Immunofluorescence - Anti-Ran antibody [EPR10791(B)] (ab155103)](https://www.abcam.com/ps/products/155/ab155103/Images/Ran-Primary-antibodies-ab155103-2.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Ran antibody [EPR10791(B)] (ab155103)Immunofluorescence analysis of HepG2 cells labeling Ran with ab155103 at 1/250 dilution.
Protocols
References
This product has been referenced in:
- Zhang H et al. Mechanisms controlling the smooth muscle cell death in progeria via down-regulation of poly(ADP-ribose) polymerase 1. Proc Natl Acad Sci U S A 111:E2261-70 (2014). Read more (PubMed: 24843141) »
