Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline. pH 7.3
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab17034 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.05 - 0.1 µg/ml. Detects a band of approximately 28-30 kDa (predicted molecular weight: 23 kDa).Can be blocked with Human RanBP1 peptide (ab23305).|
FunctionInhibits GTP exchange on Ran. Forms a Ran-GTP-RANBP1 trimeric complex. Increase GTP hydrolysis induced by the Ran GTPase activating protein RANGAP1. May act in an intracellular signaling pathway which may control the progression through the cell cycle by regulating the transport of protein and nucleic acids across the nuclear membrane.
Sequence similaritiesBelongs to the RANBP1 family.
Contains 1 RanBD1 domain.
- Information by UniProt
- HTF9A antibody
- RAN binding protein 1 antibody
- Ran specific GTPase activating protein antibody
ab17034 (3.8µg/ml) staining of paraffin embedded Human Kidney tissue following Steamed antigen retrieval with citrate buffer pH 6 and AP-staining shows pixulated cytoplasm staining in DCT.
Anti-RanBP1 antibody (ab17034) at 0.05 µg/ml + HeLa cell Lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 23 kDa
Primary Incubation 1 hour.
ab17034 at 0.1
µg/ml staining RanBP1 from HepG2 lysate (35 µg total protein per lane) by Western blot. The primary antibody was incubated for 1 hour. The blot was detected by chemiluminescence. ab17034 at 0.1µg/ml staining RanBP1 from HepG2 lysate (35µg total protein per lane) by Western blot. The primary antibody was incubated for 1 hour. The blot was detected by chemiluminescence.
ab17034 has not yet been referenced specifically in any publications.