Product nameAnti-RanBP1 antibody
See all RanBP1 primary antibodies
DescriptionRabbit polyclonal to RanBP1
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Human
Synthetic peptide within Human RanBP1 aa 1-50. The exact sequence is proprietary.
Database link: P43487
- WB: HeLa and HEK-293T whole cell lysate. IHC-P: Human colon carcinoma tissue. IP: HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab264129 in the following tested applications.
|WB||1/1000 - 1/5000.|
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/2000 - 1/10000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
FunctionInhibits GTP exchange on Ran. Forms a Ran-GTP-RANBP1 trimeric complex. Increase GTP hydrolysis induced by the Ran GTPase activating protein RANGAP1. May act in an intracellular signaling pathway which may control the progression through the cell cycle by regulating the transport of protein and nucleic acids across the nuclear membrane.
Sequence similaritiesBelongs to the RANBP1 family.
Contains 1 RanBD1 domain.
- Information by UniProt
- HTF9A antibody
- RAN binding protein 1 antibody
- Ran specific GTPase activating protein antibody
All lanes : Anti-RanBP1 antibody (ab264129) at 0.4 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Exposure time: 3 minutes
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human colon carcinoma tissue labeling RanBP1 with ab264129 at 1/5,000 dilution. Detection: DAB.
RanBP1 was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264129 at 3µg/mg lysate. Western blot was performed from the immunoprecipitate using ab264129 at 1µg/ml.
Lane 1: ab264129 IP in HeLa whole cell lysate, lot 1.
Lane 2: ab264129 IP in HeLa whole cell lysate, lot 2.
Lane 3: Control IgG.
Exposure time: 3 mins.
ab264129 has not yet been referenced specifically in any publications.