Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab2937 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 24 kDa).Can be blocked with RanBP1 peptide (ab4938).|
FunctionInhibits GTP exchange on Ran. Forms a Ran-GTP-RANBP1 trimeric complex. Increase GTP hydrolysis induced by the Ran GTPase activating protein RANGAP1. May act in an intracellular signaling pathway which may control the progression through the cell cycle by regulating the transport of protein and nucleic acids across the nuclear membrane.
Sequence similaritiesBelongs to the RANBP1 family.
Contains 1 RanBD1 domain.
- Information by UniProt
- HTF9A antibody
- RAN binding protein 1 antibody
- Ran specific GTPase activating protein antibody
All lanes : Anti-RanBP1 antibody (ab2937) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Human brain tissue lysate
Lane 3 : NIH-3T3 cell lysate
Lysates/proteins at 25 µg per lane.
All lanes : HRP conjugated anti-rabbit
Developed using the ECL technique.
Predicted band size: 24 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
This product has been referenced in:
- Prpar Mihevc S et al. TDP-43 aggregation mirrors TDP-43 knockdown, affecting the expression levels of a common set of proteins. Sci Rep 6:33996 (2016). WB, ICC/IF . Read more (PubMed: 27665936) »
- Štalekar M et al. Proteomic analyses reveal that loss of TDP-43 affects RNA processing and intracellular transport. Neuroscience 293:157-70 (2015). WB, ICC/IF . Read more (PubMed: 25743254) »