The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at 2-5µg/mg of lysate.
WB: 1/1000 - 1/5000. Detects a band of approximately 28 kDa (predicted molecular weight: 23 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Inhibits GTP exchange on Ran. Forms a Ran-GTP-RANBP1 trimeric complex. Increase GTP hydrolysis induced by the Ran GTPase activating protein RANGAP1. May act in an intracellular signaling pathway which may control the progression through the cell cycle by regulating the transport of protein and nucleic acids across the nuclear membrane.
Belongs to the RANBP1 family. Contains 1 RanBD1 domain.
Western Blot showing ab70185 at 1µg/ml staining RanBP1 in HeLa whole cell lysates immunoprecipitated using ab70185 at 5µg/0.5mg lysate (Lane 1). Lane 2: Control IgG was used for immunoprecipitation.
Detection: Chemiluminescence with an exposure time of 5