Key features and details
- Rabbit polyclonal to RanBP1
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-RanBP1 antibody
See all RanBP1 primary antibodies
DescriptionRabbit polyclonal to RanBP1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide corresponding a region between residues 1 and 50 of human RanBP1 (NP_002873.1)
- HeLa whole cell lysates.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab70185 was affinity purified using an epitope specific to RanBP1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab70185 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000 - 1/5000. Detects a band of approximately 28 kDa (predicted molecular weight: 23 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionInhibits GTP exchange on Ran. Forms a Ran-GTP-RANBP1 trimeric complex. Increase GTP hydrolysis induced by the Ran GTPase activating protein RANGAP1. May act in an intracellular signaling pathway which may control the progression through the cell cycle by regulating the transport of protein and nucleic acids across the nuclear membrane.
Sequence similaritiesBelongs to the RANBP1 family.
Contains 1 RanBD1 domain.
- Information by UniProt
- HTF9A antibody
- RAN binding protein 1 antibody
- Ran specific GTPase activating protein antibody
Lane 1 : Anti-RanBP1 antibody (ab70185) at 1 µg/ml
Lane 2 : Anti-RanBP1 antibody (ab70185) at 0.33 µg/ml
All lanes : HeLa whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 23 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutes
Western Blot showing ab70185 at 1µg/ml staining RanBP1 in HeLa whole cell lysates immunoprecipitated using ab70185 at 5µg/0.5mg lysate (Lane 1). Lane 2: Control IgG was used for immunoprecipitation. Detection: Chemiluminescence with an exposure time of 5 minutes
ab70185 has not yet been referenced specifically in any publications.