Anti-RanBP1 antibody (ab97659)
Key features and details
- Rabbit polyclonal to RanBP1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RanBP1 antibody
See all RanBP1 primary antibodies -
Description
Rabbit polyclonal to RanBP1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow
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Immunogen
Recombinant fragment, corresponding to a sequence within amino acids 1-197 of Human RanBP1 (NP_002873).
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Positive control
- 293T, A431, H1299, HeLaS3, HepG2, MOLT4 and Raji cell lysates; HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97659 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 23 kDa.
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| ICC/IF |
1/100 - 1/200.
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| Notes |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 23 kDa. |
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ICC/IF
1/100 - 1/200. |
Target
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Function
Inhibits GTP exchange on Ran. Forms a Ran-GTP-RANBP1 trimeric complex. Increase GTP hydrolysis induced by the Ran GTPase activating protein RANGAP1. May act in an intracellular signaling pathway which may control the progression through the cell cycle by regulating the transport of protein and nucleic acids across the nuclear membrane. -
Sequence similarities
Belongs to the RANBP1 family.
Contains 1 RanBD1 domain. - Information by UniProt
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Database links
- Entrez Gene: 533251 Cow
- Entrez Gene: 5902 Human
- Entrez Gene: 19385 Mouse
- Entrez Gene: 360739 Rat
- Omim: 601180 Human
- SwissProt: Q3T0M7 Cow
- SwissProt: P43487 Human
- SwissProt: P34022 Mouse
see all -
Alternative names
- HTF9A antibody
- RAN binding protein 1 antibody
- Ran specific GTPase activating protein antibody
see all
Images
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Western blot - Anti-RanBP1 antibody (ab97659)Anti-RanBP1 antibody (ab97659) at 1/1000 dilution + HeLa S3 whole cell lysate at 30 µg
Predicted band size: 23 kDa
12% SDS-PAGE. -
Immunocytochemistry/ Immunofluorescence - Anti-RanBP1 antibody (ab97659)ab97659 at 1/200 dilution staining RanBP1 in HeLa cells by Immunofluorescence, Paraformaldehyde fixed. Lower image is merged with DNA probe.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab97659 has been referenced in 6 publications.
- Miloudi H et al. STAT6 is a cargo of exportin 1: Biological relevance in primary mediastinal B-cell lymphoma. Cell Signal 46:76-82 (2018). PubMed: 29501729
- Mencarelli C et al. RanBP1 Couples Nuclear Export and Golgi Regulation through LKB1 to Promote Cortical Neuron Polarity. Cell Rep 24:2529-2539.e4 (2018). PubMed: 30184488
- Andresen V et al. Anti-proliferative activity of the NPM1 interacting natural product avrainvillamide in acute myeloid leukemia. Cell Death Dis 7:e2497 (2016). WB ; Human . PubMed: 27906185
- Neggers JE et al. Heterozygous mutation of cysteine528 in XPO1 is sufficient for resistance to selective inhibitors of nuclear export. Oncotarget 7:68842-68850 (2016). PubMed: 27634897
- Neggers JE et al. Identifying drug-target selectivity of small-molecule CRM1/XPO1 inhibitors by CRISPR/Cas9 genome editing. Chem Biol 22:107-16 (2015). ICC/IF ; Human . PubMed: 25579209
- Hwang HI et al. Phosphorylation of Ran-binding Protein-1 by Polo-like Kinase-1 Is Required for Interaction with Ran and Early Mitotic Progression. J Biol Chem 286:33012-20 (2011). WB, ICC/IF ; Human . PubMed: 21813642