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Cell Biology Cell Cycle Cell Division Spindle
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Anti-RanBP1 antibody (ab97659)

  • Datasheet
  • SDS
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Western blot - Anti-RanBP1 antibody (ab97659)
  • Immunocytochemistry/ Immunofluorescence - Anti-RanBP1 antibody (ab97659)

Key features and details

  • Rabbit polyclonal to RanBP1
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Human RANBP1 knockout HEK-293T cell line (ab266202)

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Overview

  • Product name

    Anti-RanBP1 antibody
    See all RanBP1 primary antibodies
  • Description

    Rabbit polyclonal to RanBP1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment, corresponding to a sequence within amino acids 1-197 of Human RanBP1 (NP_002873).

  • Positive control

    • 293T, A431, H1299, HeLaS3, HepG2, MOLT4 and Raji cell lysates; HeLa cells.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Spindle
    • Signal Transduction
    • Protein Trafficking
    • Nuclear Import / Export
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Other

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa S3 nuclear extract lysate (ab14654)
    • Hep G2 cytoplasmic extract lysate (ab14659)
    • Hep G2 nuclear extract lysate (ab14660)
    • Raji whole cell lysate (ab30124)
  • Recombinant Protein

    • Recombinant Human RanBP1 protein (ab124580)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab97659 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/500 - 1/3000. Predicted molecular weight: 23 kDa.
ICC/IF
1/100 - 1/200.
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 23 kDa.
ICC/IF
1/100 - 1/200.

Target

  • Function

    Inhibits GTP exchange on Ran. Forms a Ran-GTP-RANBP1 trimeric complex. Increase GTP hydrolysis induced by the Ran GTPase activating protein RANGAP1. May act in an intracellular signaling pathway which may control the progression through the cell cycle by regulating the transport of protein and nucleic acids across the nuclear membrane.
  • Sequence similarities

    Belongs to the RANBP1 family.
    Contains 1 RanBD1 domain.
  • Target information above from: UniProt accession P43487 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 533251 Cow
    • Entrez Gene: 5902 Human
    • Entrez Gene: 19385 Mouse
    • Entrez Gene: 360739 Rat
    • Omim: 601180 Human
    • SwissProt: Q3T0M7 Cow
    • SwissProt: P43487 Human
    • SwissProt: P34022 Mouse
    • Unigene: 24763 Human
    • Unigene: 235287 Mouse
    see all
  • Alternative names

    • HTF9A antibody
    • RAN binding protein 1 antibody
    • Ran specific GTPase activating protein antibody
    • Ran-binding protein 1 antibody
    • Ran-specific GTPase-activating protein antibody
    • RANBP1 antibody
    • RANG_HUMAN antibody
    see all

Images

  • Western blot - Anti-RanBP1 antibody (ab97659)
    Western blot - Anti-RanBP1 antibody (ab97659)
    Anti-RanBP1 antibody (ab97659) at 1/1000 dilution + HeLa S3 whole cell lysate at 30 µg

    Predicted band size: 23 kDa



    12% SDS-PAGE.
  • Immunocytochemistry/ Immunofluorescence - Anti-RanBP1 antibody (ab97659)
    Immunocytochemistry/ Immunofluorescence - Anti-RanBP1 antibody (ab97659)
    ab97659 at 1/200 dilution staining RanBP1 in HeLa cells by Immunofluorescence, Paraformaldehyde fixed. Lower image is merged with DNA probe.

Protocols

  • Western blot protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (6)

Publishing research using ab97659? Please let us know so that we can cite the reference in this datasheet.

ab97659 has been referenced in 6 publications.

  • Miloudi H  et al. STAT6 is a cargo of exportin 1: Biological relevance in primary mediastinal B-cell lymphoma. Cell Signal 46:76-82 (2018). PubMed: 29501729
  • Mencarelli C  et al. RanBP1 Couples Nuclear Export and Golgi Regulation through LKB1 to Promote Cortical Neuron Polarity. Cell Rep 24:2529-2539.e4 (2018). PubMed: 30184488
  • Andresen V  et al. Anti-proliferative activity of the NPM1 interacting natural product avrainvillamide in acute myeloid leukemia. Cell Death Dis 7:e2497 (2016). WB ; Human . PubMed: 27906185
  • Neggers JE  et al. Heterozygous mutation of cysteine528 in XPO1 is sufficient for resistance to selective inhibitors of nuclear export. Oncotarget 7:68842-68850 (2016). PubMed: 27634897
  • Neggers JE  et al. Identifying drug-target selectivity of small-molecule CRM1/XPO1 inhibitors by CRISPR/Cas9 genome editing. Chem Biol 22:107-16 (2015). ICC/IF ; Human . PubMed: 25579209
  • Hwang HI  et al. Phosphorylation of Ran-binding Protein-1 by Polo-like Kinase-1 Is Required for Interaction with Ran and Early Mitotic Progression. J Biol Chem 286:33012-20 (2011). WB, ICC/IF ; Human . PubMed: 21813642

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