Overview

  • Product name

    Anti-RANBP10 antibody
  • Description

    Rabbit polyclonal to RANBP10
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Cow
  • Immunogen

    Recombinant fragment corresponding to Human RANBP10 aa 3-165.
    Database link: Q6VN20

  • Positive control

    • WB: SH-SY5Y, HeLa and MCF7 whole cell lysate. IHC-P: Human skeletal muscle tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.4
    Preservative: 0.03% Proclin
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purity >95%.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab235104 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/500.
WB 1/500 - 1/5000.

Target

  • Function

    May act as an adapter protein to couple membrane receptors to intracellular signaling pathways. In contrast to RANBP9, does not interact with Sos and does not activate the Ras pathway.
  • Tissue specificity

    Broadly expressed, with highest levels in skeletal muscle.
  • Sequence similarities

    Belongs to the RANBP9/10 family.
    Contains 1 B30.2/SPRY domain.
    Contains 1 CTLH domain.
    Contains 1 LisH domain.
  • Domain

    The SPRY domain mediates the interaction with MET.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ31165 antibody
    • KIAA1464 antibody
    • RAN binding protein 10 antibody
    • Ran-binding protein 10 antibody
    • RanBP10 antibody
    • RBP10_HUMAN antibody
    see all

Images

  • Paraffin-embedded human skeletal muscle tissue stained for RANBP10 with ab235104 at 1/200 dilution in immunohistochemical analysis.

    After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at room temperature. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.

  • All lanes : Anti-RANBP10 antibody (ab235104) at 1/500 dilution

    Lane 1 : SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate
    Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 3 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate

    Secondary
    All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution

References

ab235104 has not yet been referenced specifically in any publications.

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