Key features and details
- Rabbit polyclonal to RANBP10
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-RANBP10 antibody
DescriptionRabbit polyclonal to RANBP10
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Cow
Recombinant fragment corresponding to Human RANBP10 aa 3-165.
Database link: Q6VN20
- WB: SH-SY5Y, HeLa and MCF7 whole cell lysate. IHC-P: Human skeletal muscle tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab235104 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200 - 1/500.|
|WB||1/500 - 1/5000.|
FunctionMay act as an adapter protein to couple membrane receptors to intracellular signaling pathways. In contrast to RANBP9, does not interact with Sos and does not activate the Ras pathway.
Tissue specificityBroadly expressed, with highest levels in skeletal muscle.
Sequence similaritiesBelongs to the RANBP9/10 family.
Contains 1 B30.2/SPRY domain.
Contains 1 CTLH domain.
Contains 1 LisH domain.
DomainThe SPRY domain mediates the interaction with MET.
- Information by UniProt
- FLJ31165 antibody
- KIAA1464 antibody
- RAN binding protein 10 antibody
Paraffin-embedded human skeletal muscle tissue stained for RANBP10 with ab235104 at 1/200 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at room temperature. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.
All lanes : Anti-RANBP10 antibody (ab235104) at 1/500 dilution
Lane 1 : SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab235104 has not yet been referenced specifically in any publications.