Overview

  • Product name

    Anti-RanBP17 antibody
  • Description

    Rabbit polyclonal to RanBP17
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat, Guinea pig, Cow, Dog, Pig, Chimpanzee, Ferret, Chinese hamster
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 1000-1050 of Human RanBP17 (NP_075048.1).

  • Positive control

    • HeLa, 293T and Jurkat whole cell lysate (ab7899).

Properties

Applications

Our Abpromise guarantee covers the use of ab168842 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 124 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

Images

  • All lanes : Anti-RanBP17 antibody (ab168842) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate
    Lane 2 : 293T whole cell lysate
    Lane 3 : Jurkat whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 124 kDa


    Exposure time: 3 minutes
  • Detection of RanBP17 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab168842 at 6 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
    Detection: Chemiluminescence with an exposure time of 30 seconds.

References

ab168842 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab168842.
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