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    ranbp2-antibody-ab112061.pdf

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Cell Biology Cell Cycle Cell Division Spindle
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Anti-RanBP2 antibody (ab112061)

  • Datasheet
  • SDS
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Western blot - Anti-RanBP2 antibody (ab112061)
  • Immunoprecipitation - Anti-RanBP2 antibody (ab112061)

Key features and details

  • Rabbit polyclonal to RanBP2
  • Suitable for: WB, IP
  • Reacts with: Human
  • Isotype: IgG

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Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-RanBP2 antibody
    See all RanBP2 primary antibodies
  • Description

    Rabbit polyclonal to RanBP2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 1050-1100 of Human RanBP2 (NP_006258.3).

  • Positive control

    • HeLa Whole cell lysate.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 99% Tris buffered saline, 0.1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab112061 was affinity purified using an epitope specific to RanBP2 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Spindle
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Sumo
    • Signal Transduction
    • Protein Trafficking
    • Nuclear Import / Export
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Other
    • Epigenetics and Nuclear Signaling
    • Ubiquitin & Ubiquitin Like Modifiers
    • Ubiquitin like Modifiers

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab112061 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/2000 - 1/10000. Predicted molecular weight: 358 kDa.
IP
Use at 2-5 µg/mg of lysate.
Notes
WB
1/2000 - 1/10000. Predicted molecular weight: 358 kDa.
IP
Use at 2-5 µg/mg of lysate.

Target

  • Function

    E3 SUMO-protein ligase which facilitates SUMO1 and SUMO2 conjugation by UBE2I. Involved in transport factor (Ran-GTP, karyopherin)-mediated protein import via the F-G repeat-containing domain which acts as a docking site for substrates. Could also have isomerase or chaperone activity and may bind RNA or DNA. Component of the nuclear export pathway. Specific docking site for the nuclear export factor exportin-1.
  • Pathway

    Protein modification; protein sumoylation.
  • Involvement in disease

    Defects in RANBP2 are the cause of susceptibility to encephalopathy acute necrotizing type 1 (ANE1) [MIM:608033]. A rapidly progressive encephalopathy manifesting in susceptibile individuals with seizures and coma. It can occur within days in otherwise healthy children after common viral infections such as influenza and parainfluenza, without evidence of viral infection of the brain or inflammatory cell infiltration. Brain T2-weighted magnetic resonance imaging reveals characteristic symmetric lesions present in the thalami, pons and brainstem.
  • Sequence similarities

    Contains 1 PPIase cyclophilin-type domain.
    Contains 4 RanBD1 domains.
    Contains 8 RanBP2-type zinc fingers.
    Contains 1 TPR repeat.
  • Domain

    Contains F-X-F-G repeats.
  • Post-translational
    modifications

    Polyubiquitinated by PARK2, which leads to proteasomal degradation.
  • Cellular localization

    Nucleus > nuclear pore complex. Cytoplasmic filaments.
  • Target information above from: UniProt accession P49792 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5903 Human
    • Omim: 601181 Human
    • SwissProt: P49792 Human
    • Unigene: 199561 Human
    • Unigene: 715056 Human
    • Alternative names

      • 358 kDa nucleoporin antibody
      • ANE1 antibody
      • E3 SUMO-protein ligase RanBP2 antibody
      • IIAE3 antibody
      • Nuclear pore complex protein Nup358 antibody
      • Nucleoporin 358 antibody
      • Nucleoporin Nup358 antibody
      • NUP358 antibody
      • p270 antibody
      • RAN binding protein 2 antibody
      • Ran-binding protein 2 antibody
      • RANBP2 antibody
      • RBP2_HUMAN antibody
      • Transformation related protein 2 antibody
      • TRP 1 antibody
      • TRP 2 antibody
      • TRP1 antibody
      • TRP2 antibody
      see all

    Images

    • Western blot - Anti-RanBP2 antibody (ab112061)
      Western blot - Anti-RanBP2 antibody (ab112061)
      All lanes : Anti-RanBP2 antibody (ab112061) at 0.04 µg/ml

      Lane 1 : Hela whole cell lysate at 50 µg
      Lane 2 : Hela whole cell lysate at 15 µg
      Lane 3 : Hela whole cell lysate at 5 µg

      Developed using the ECL technique.

      Predicted band size: 358 kDa


      Exposure time: 10 seconds
    • Immunoprecipitation - Anti-RanBP2 antibody (ab112061)
      Immunoprecipitation - Anti-RanBP2 antibody (ab112061)
      Detection of RanBP2 by Western Blot of Immunprecipitate.
      ab112061 at 0.1µg/ml staining RanBP2 in HeLa whole cell lysate immunoprecipitated using ab112061 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
      Detection: Chemiluminescence with exposure time of 3 seconds.

    Protocols

    • Western blot protocols
    • Immunoprecipitation protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab112061? Please let us know so that we can cite the reference in this datasheet.

    ab112061 has not yet been referenced specifically in any publications.

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