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- Specific References
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Overview
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Product nameAnti-RanBP2 antibody
See all RanBP2 primary antibodies -
DescriptionRabbit polyclonal to RanBP2
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IPmore details
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Species reactivityReacts with: Human
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Immunogen
Synthetic peptide, corresponding to a region within amino acids 1050-1100 of Human RanBP2 (NP_006258.3).
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Positive control
- HeLa Whole cell lysate.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesab112061 was affinity purified using an epitope specific to RanBP2 immobilized on solid support.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab112061 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000 - 1/10000. Predicted molecular weight: 358 kDa. | |
| IP | Use at 2-5 µg/mg of lysate. |
Target
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FunctionE3 SUMO-protein ligase which facilitates SUMO1 and SUMO2 conjugation by UBE2I. Involved in transport factor (Ran-GTP, karyopherin)-mediated protein import via the F-G repeat-containing domain which acts as a docking site for substrates. Could also have isomerase or chaperone activity and may bind RNA or DNA. Component of the nuclear export pathway. Specific docking site for the nuclear export factor exportin-1.
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PathwayProtein modification; protein sumoylation.
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Involvement in diseaseDefects in RANBP2 are the cause of susceptibility to encephalopathy acute necrotizing type 1 (ANE1) [MIM:608033]. A rapidly progressive encephalopathy manifesting in susceptibile individuals with seizures and coma. It can occur within days in otherwise healthy children after common viral infections such as influenza and parainfluenza, without evidence of viral infection of the brain or inflammatory cell infiltration. Brain T2-weighted magnetic resonance imaging reveals characteristic symmetric lesions present in the thalami, pons and brainstem.
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Sequence similaritiesContains 1 PPIase cyclophilin-type domain.
Contains 4 RanBD1 domains.
Contains 8 RanBP2-type zinc fingers.
Contains 1 TPR repeat. -
DomainContains F-X-F-G repeats.
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Post-translational
modificationsPolyubiquitinated by PARK2, which leads to proteasomal degradation. -
Cellular localizationNucleus > nuclear pore complex. Cytoplasmic filaments.
- Information by UniProt
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Database links
- Entrez Gene: 5903 Human
- Omim: 601181 Human
- SwissProt: P49792 Human
- Unigene: 199561 Human
- Unigene: 715056 Human
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Alternative names
- 358 kDa nucleoporin antibody
- ANE1 antibody
- E3 SUMO-protein ligase RanBP2 antibody
see all
Images
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Western blot - Anti-RanBP2 antibody (ab112061)All lanes : Anti-RanBP2 antibody (ab112061) at 0.04 µg/ml
Lane 1 : Hela whole cell lysate at 50 µg
Lane 2 : Hela whole cell lysate at 15 µg
Lane 3 : Hela whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 358 kDa
Exposure time: 10 seconds -
Immunoprecipitation - Anti-RanBP2 antibody (ab112061)Detection of RanBP2 by Western Blot of Immunprecipitate.
ab112061 at 0.1µg/ml staining RanBP2 in HeLa whole cell lysate immunoprecipitated using ab112061 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 3 seconds.
References
ab112061 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
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