Overview

  • Product name

  • Description

    Rabbit polyclonal to RanBP2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee
  • Immunogen

    Synthetic peptide within Human RanBP2 aa 2600-2650. The exact sequence is proprietary. NP_006258.3
    Database link: P49792

  • Positive control

    • WB: HeLa whole cell lysate. IP: RanBP2 IP in HeLa whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab245563 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000.
IP Use at 2-5 µg/mg of lysate.

Target

  • Function

    E3 SUMO-protein ligase which facilitates SUMO1 and SUMO2 conjugation by UBE2I. Involved in transport factor (Ran-GTP, karyopherin)-mediated protein import via the F-G repeat-containing domain which acts as a docking site for substrates. Could also have isomerase or chaperone activity and may bind RNA or DNA. Component of the nuclear export pathway. Specific docking site for the nuclear export factor exportin-1.
  • Pathway

    Protein modification; protein sumoylation.
  • Involvement in disease

    Defects in RANBP2 are the cause of susceptibility to encephalopathy acute necrotizing type 1 (ANE1) [MIM:608033]. A rapidly progressive encephalopathy manifesting in susceptibile individuals with seizures and coma. It can occur within days in otherwise healthy children after common viral infections such as influenza and parainfluenza, without evidence of viral infection of the brain or inflammatory cell infiltration. Brain T2-weighted magnetic resonance imaging reveals characteristic symmetric lesions present in the thalami, pons and brainstem.
  • Sequence similarities

    Contains 1 PPIase cyclophilin-type domain.
    Contains 4 RanBD1 domains.
    Contains 8 RanBP2-type zinc fingers.
    Contains 1 TPR repeat.
  • Domain

    Contains F-X-F-G repeats.
  • Post-translational
    modifications

    Polyubiquitinated by PARK2, which leads to proteasomal degradation.
  • Cellular localization

    Nucleus > nuclear pore complex. Cytoplasmic filaments.
  • Information by UniProt
  • Database links

  • Alternative names

    • 358 kDa nucleoporin antibody
    • ANE1 antibody
    • E3 SUMO-protein ligase RanBP2 antibody
    • IIAE3 antibody
    • Nuclear pore complex protein Nup358 antibody
    • Nucleoporin 358 antibody
    • Nucleoporin Nup358 antibody
    • NUP358 antibody
    • p270 antibody
    • RAN binding protein 2 antibody
    • Ran-binding protein 2 antibody
    • RANBP2 antibody
    • RBP2_HUMAN antibody
    • Transformation related protein 2 antibody
    • TRP 1 antibody
    • TRP 2 antibody
    • TRP1 antibody
    • TRP2 antibody
    see all

Images

  • All lanes : Anti-RanBP2 antibody (ab245563) at 0.04 µg/ml

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Exposure time: 5 seconds
  • RanBP2 was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded).

    ab245563 used for IP at 3 µg/mg lysate. For WB 0.1 µg/ml.

    Lane 1: ab245563 IP in HeLa whole cell lysate.
    Lane 2: Control IgG in HeLa whole cell lysate.
    Chemiluminescence detection: 1 second.

References

ab245563 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab245563.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up