Overview

  • Product name

  • Description

    Rabbit polyclonal to RanBP2
  • Host species

    Rabbit
  • Specificity

    Detects Ran binding protein 2 (RanBP 2) from human and bovine retina.
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Cow, Human
    Predicted to work with: Chimpanzee
  • Immunogen

    Synthetic peptide corresponding to Human RanBP2 aa 1-18.
    Sequence:

    MRRSKADVERYIASVQGS


    (Peptide available as ab4939)

  • Positive control

    • Bovine retinal extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab2938 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 µg/ml.

Immunohistochemical staining of bovine retina results in cytoplasmic staining of cells within the inner nuclear layer as well as the ganglion cells.

WB Use a concentration of 1 µg/ml. Detects a band of approximately 360 kDa (predicted molecular weight: 358 kDa).Can be blocked with RanBP2 peptide (ab4939).

Detects a band of approximately 360 kDa representing RanBP 2 from bovine retinal extracts.

Target

  • Function

    E3 SUMO-protein ligase which facilitates SUMO1 and SUMO2 conjugation by UBE2I. Involved in transport factor (Ran-GTP, karyopherin)-mediated protein import via the F-G repeat-containing domain which acts as a docking site for substrates. Could also have isomerase or chaperone activity and may bind RNA or DNA. Component of the nuclear export pathway. Specific docking site for the nuclear export factor exportin-1.
  • Pathway

    Protein modification; protein sumoylation.
  • Involvement in disease

    Defects in RANBP2 are the cause of susceptibility to encephalopathy acute necrotizing type 1 (ANE1) [MIM:608033]. A rapidly progressive encephalopathy manifesting in susceptibile individuals with seizures and coma. It can occur within days in otherwise healthy children after common viral infections such as influenza and parainfluenza, without evidence of viral infection of the brain or inflammatory cell infiltration. Brain T2-weighted magnetic resonance imaging reveals characteristic symmetric lesions present in the thalami, pons and brainstem.
  • Sequence similarities

    Contains 1 PPIase cyclophilin-type domain.
    Contains 4 RanBD1 domains.
    Contains 8 RanBP2-type zinc fingers.
    Contains 1 TPR repeat.
  • Domain

    Contains F-X-F-G repeats.
  • Post-translational
    modifications

    Polyubiquitinated by PARK2, which leads to proteasomal degradation.
  • Cellular localization

    Nucleus > nuclear pore complex. Cytoplasmic filaments.
  • Information by UniProt
  • Database links

  • Alternative names

    • 358 kDa nucleoporin antibody
    • ANE1 antibody
    • E3 SUMO-protein ligase RanBP2 antibody
    • IIAE3 antibody
    • Nuclear pore complex protein Nup358 antibody
    • Nucleoporin 358 antibody
    • Nucleoporin Nup358 antibody
    • NUP358 antibody
    • p270 antibody
    • RAN binding protein 2 antibody
    • Ran-binding protein 2 antibody
    • RANBP2 antibody
    • RBP2_HUMAN antibody
    • Transformation related protein 2 antibody
    • TRP 1 antibody
    • TRP 2 antibody
    • TRP1 antibody
    • TRP2 antibody
    see all

Images

  • Immunohistochemical staining of RanBP2 in bovine retina using ab2938.

  • Western blot of RanBP2 from human retinal extracts using ab2938. Western blot of RanBP2 from human retinal extracts using ab2938.

References

This product has been referenced in:

  • Li H  et al. Nucleolar and spindle associated protein 1 promotes metastasis of cervical carcinoma cells by activating Wnt/ß-catenin signaling. J Exp Clin Cancer Res 38:33 (2019). Read more (PubMed: 30678687) »
  • Saito A  et al. Roles of Capsid-Interacting Host Factors in Multimodal Inhibition of HIV-1 by PF74. J Virol 90:5808-23 (2016). Read more (PubMed: 27076642) »
See all 9 Publications for this product

Customer reviews and Q&As

Answer

This was done on cryosections fixed in 2% paraformaldehyde and permeabilized with Triton X-100 (or saponin can be used).

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up