Anti-RanBP2 antibody (ab64276)
Key features and details
- Rabbit polyclonal to RanBP2
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RanBP2 antibody
See all RanBP2 primary antibodies -
Description
Rabbit polyclonal to RanBP2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Synthetic peptide (human) corresponding to 3 times repeated sequence in RanBP2 protein:
SKAPKSGFEGMFTKKE
, amino acids 1592-1607. -
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6
Preservative: 0.09% Sodium azide
Constituent: Whole serum -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab64276 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC/IF |
Human
|
| WB |
Human
|
| All applications |
Cow
|
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/1000. Detects a band of approximately 358 kDa (predicted molecular weight: 358 kDa).
|
| ICC/IF | (3) |
1/2000.
|
| Notes |
|---|
|
WB
1/1000. Detects a band of approximately 358 kDa (predicted molecular weight: 358 kDa). |
|
ICC/IF
1/2000. |
Target
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Function
E3 SUMO-protein ligase which facilitates SUMO1 and SUMO2 conjugation by UBE2I. Involved in transport factor (Ran-GTP, karyopherin)-mediated protein import via the F-G repeat-containing domain which acts as a docking site for substrates. Could also have isomerase or chaperone activity and may bind RNA or DNA. Component of the nuclear export pathway. Specific docking site for the nuclear export factor exportin-1. -
Pathway
Protein modification; protein sumoylation. -
Involvement in disease
Defects in RANBP2 are the cause of susceptibility to encephalopathy acute necrotizing type 1 (ANE1) [MIM:608033]. A rapidly progressive encephalopathy manifesting in susceptibile individuals with seizures and coma. It can occur within days in otherwise healthy children after common viral infections such as influenza and parainfluenza, without evidence of viral infection of the brain or inflammatory cell infiltration. Brain T2-weighted magnetic resonance imaging reveals characteristic symmetric lesions present in the thalami, pons and brainstem. -
Sequence similarities
Contains 1 PPIase cyclophilin-type domain.
Contains 4 RanBD1 domains.
Contains 8 RanBP2-type zinc fingers.
Contains 1 TPR repeat. -
Domain
Contains F-X-F-G repeats. -
Post-translational
modificationsPolyubiquitinated by PARK2, which leads to proteasomal degradation. -
Cellular localization
Nucleus > nuclear pore complex. Cytoplasmic filaments. - Information by UniProt
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Database links
- Entrez Gene: 785768 Cow
- Entrez Gene: 5903 Human
- Omim: 601181 Human
- SwissProt: P48820 Cow
- SwissProt: P49792 Human
- Unigene: 199561 Human
- Unigene: 715056 Human
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Alternative names
- 358 kDa nucleoporin antibody
- ANE1 antibody
- E3 SUMO-protein ligase RanBP2 antibody
see all
Images
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Western blot - Anti-RanBP2 antibody (ab64276)Anti-RanBP2 antibody (ab64276) at 1/1000 dilution + HeLa total cell lysate
Predicted band size: 358 kDa
Observed band size: 358 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-RanBP2 antibody (ab64276)ab64276 (1:2000) staining RanBP2 in Hela cells pre-extracted in 0.005% digitonin and fixed in 3.7% PFA/30 mM sucrose/PBS for 15 min (room temperature). Image kindly provided by Patrizia Lavia, Univ. "La Sapienza", CNR, Italy. -
Immunocytochemistry/ Immunofluorescence - Anti-RanBP2 antibody (ab64276)Immunofluorescent staining of paraformaldehyde fixed HeLa cells by ab64276 (1:2000). -
Western blot - Anti-RanBP2 antibody (ab64276)Anti-RanBP2 antibody (ab64276) at 1/2000 dilution + Hela whole cell lysate
Predicted band size: 358 kDa
Exposure time: 1 minute
WB performed on a 6%SDS-PAGE gel.Image Kindly provided by Patrizia Lavia, University "La Sapienza", CNR, Italy.
Protocols
Datasheets and documents
References (20)
ab64276 has been referenced in 20 publications.
- Bentz GL et al. Using glycyrrhizic acid to target sumoylation processes during Epstein-Barr virus latency. PLoS One 14:e0217578 (2019). PubMed: 31125383
- Yan P et al. FOXO3-Engineered Human ESC-Derived Vascular Cells Promote Vascular Protection and Regeneration. Cell Stem Cell 24:447-461.e8 (2019). PubMed: 30661960
- Ben-Yishay R et al. Imaging within single NPCs reveals NXF1's role in mRNA export on the cytoplasmic side of the pore. J Cell Biol 218:2962-2981 (2019). PubMed: 31375530
- Francis AC & Melikyan GB Single HIV-1 Imaging Reveals Progression of Infection through CA-Dependent Steps of Docking at the Nuclear Pore, Uncoating, and Nuclear Transport. Cell Host Microbe 23:536-548.e6 (2018). PubMed: 29649444
- Kane M et al. Nuclear pore heterogeneity influences HIV-1 infection and the antiviral activity of MX2. Elife 7:N/A (2018). PubMed: 30084827
