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    ranbp2-antibody-ab64276.pdf

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Anti-RanBP2 antibody (ab64276)

  • Datasheet
Reviews (4) Submit a question References (20)

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Western blot - Anti-RanBP2 antibody (ab64276)
  • Immunocytochemistry/ Immunofluorescence - Anti-RanBP2 antibody (ab64276)
  • Immunocytochemistry/ Immunofluorescence - Anti-RanBP2 antibody (ab64276)
  • Western blot - Anti-RanBP2 antibody (ab64276)

Key features and details

  • Rabbit polyclonal to RanBP2
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-RanBP2 antibody
    See all RanBP2 primary antibodies
  • Description

    Rabbit polyclonal to RanBP2
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide (human) corresponding to 3 times repeated sequence in RanBP2 protein:

    SKAPKSGFEGMFTKKE

    , amino acids 1592-1607.
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 6
    Preservative: 0.09% Sodium azide
    Constituent: Whole serum
  • Concentration information loading...
  • Purity

    Whole antiserum
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Spindle
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Sumo
    • Signal Transduction
    • Protein Trafficking
    • Nuclear Import / Export
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Other
    • Epigenetics and Nuclear Signaling
    • Ubiquitin & Ubiquitin Like Modifiers
    • Ubiquitin like Modifiers

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab64276 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
WB
Human
All applications
Cow
Application Abreviews Notes
WB (1)
1/1000. Detects a band of approximately 358 kDa (predicted molecular weight: 358 kDa).
ICC/IF (3)
1/2000.
Notes
WB
1/1000. Detects a band of approximately 358 kDa (predicted molecular weight: 358 kDa).
ICC/IF
1/2000.

Target

  • Function

    E3 SUMO-protein ligase which facilitates SUMO1 and SUMO2 conjugation by UBE2I. Involved in transport factor (Ran-GTP, karyopherin)-mediated protein import via the F-G repeat-containing domain which acts as a docking site for substrates. Could also have isomerase or chaperone activity and may bind RNA or DNA. Component of the nuclear export pathway. Specific docking site for the nuclear export factor exportin-1.
  • Pathway

    Protein modification; protein sumoylation.
  • Involvement in disease

    Defects in RANBP2 are the cause of susceptibility to encephalopathy acute necrotizing type 1 (ANE1) [MIM:608033]. A rapidly progressive encephalopathy manifesting in susceptibile individuals with seizures and coma. It can occur within days in otherwise healthy children after common viral infections such as influenza and parainfluenza, without evidence of viral infection of the brain or inflammatory cell infiltration. Brain T2-weighted magnetic resonance imaging reveals characteristic symmetric lesions present in the thalami, pons and brainstem.
  • Sequence similarities

    Contains 1 PPIase cyclophilin-type domain.
    Contains 4 RanBD1 domains.
    Contains 8 RanBP2-type zinc fingers.
    Contains 1 TPR repeat.
  • Domain

    Contains F-X-F-G repeats.
  • Post-translational
    modifications

    Polyubiquitinated by PARK2, which leads to proteasomal degradation.
  • Cellular localization

    Nucleus > nuclear pore complex. Cytoplasmic filaments.
  • Target information above from: UniProt accession P49792 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 785768 Cow
    • Entrez Gene: 5903 Human
    • Omim: 601181 Human
    • SwissProt: P48820 Cow
    • SwissProt: P49792 Human
    • Unigene: 199561 Human
    • Unigene: 715056 Human
    • Alternative names

      • 358 kDa nucleoporin antibody
      • ANE1 antibody
      • E3 SUMO-protein ligase RanBP2 antibody
      • IIAE3 antibody
      • Nuclear pore complex protein Nup358 antibody
      • Nucleoporin 358 antibody
      • Nucleoporin Nup358 antibody
      • NUP358 antibody
      • p270 antibody
      • RAN binding protein 2 antibody
      • Ran-binding protein 2 antibody
      • RANBP2 antibody
      • RBP2_HUMAN antibody
      • Transformation related protein 2 antibody
      • TRP 1 antibody
      • TRP 2 antibody
      • TRP1 antibody
      • TRP2 antibody
      see all

    Images

    • Western blot - Anti-RanBP2 antibody (ab64276)
      Western blot - Anti-RanBP2 antibody (ab64276)
      Anti-RanBP2 antibody (ab64276) at 1/1000 dilution + HeLa total cell lysate

      Predicted band size: 358 kDa
      Observed band size: 358 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-RanBP2 antibody (ab64276)
      Immunocytochemistry/ Immunofluorescence - Anti-RanBP2 antibody (ab64276)
      ab64276 (1:2000) staining RanBP2 in Hela cells pre-extracted in 0.005% digitonin and fixed in 3.7% PFA/30 mM sucrose/PBS for 15 min (room temperature). Image kindly provided by Patrizia Lavia, Univ. "La Sapienza", CNR, Italy.
    • Immunocytochemistry/ Immunofluorescence - Anti-RanBP2 antibody (ab64276)
      Immunocytochemistry/ Immunofluorescence - Anti-RanBP2 antibody (ab64276)
      Immunofluorescent staining of paraformaldehyde fixed HeLa cells by ab64276 (1:2000).
    • Western blot - Anti-RanBP2 antibody (ab64276)
      Western blot - Anti-RanBP2 antibody (ab64276)
      Anti-RanBP2 antibody (ab64276) at 1/2000 dilution + Hela whole cell lysate

      Predicted band size: 358 kDa


      Exposure time: 1 minute


      WB performed on a 6%SDS-PAGE gel.

      Image Kindly provided by Patrizia Lavia, University "La Sapienza", CNR, Italy.

    Protocols

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (20)

    Publishing research using ab64276? Please let us know so that we can cite the reference in this datasheet.

    ab64276 has been referenced in 20 publications.

    • Bentz GL  et al. Using glycyrrhizic acid to target sumoylation processes during Epstein-Barr virus latency. PLoS One 14:e0217578 (2019). PubMed: 31125383
    • Yan P  et al. FOXO3-Engineered Human ESC-Derived Vascular Cells Promote Vascular Protection and Regeneration. Cell Stem Cell 24:447-461.e8 (2019). PubMed: 30661960
    • Ben-Yishay R  et al. Imaging within single NPCs reveals NXF1's role in mRNA export on the cytoplasmic side of the pore. J Cell Biol 218:2962-2981 (2019). PubMed: 31375530
    • Francis AC & Melikyan GB Single HIV-1 Imaging Reveals Progression of Infection through CA-Dependent Steps of Docking at the Nuclear Pore, Uncoating, and Nuclear Transport. Cell Host Microbe 23:536-548.e6 (2018). PubMed: 29649444
    • Kane M  et al. Nuclear pore heterogeneity influences HIV-1 infection and the antiviral activity of MX2. Elife 7:N/A (2018). PubMed: 30084827
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-4 of 4 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-RanBP2 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Permeabilization
    Yes - 0.2% Tween
    Specification
    HeLa
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 21°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 09 2018

    Immunocytochemistry/ Immunofluorescence abreview for Anti-RanBP2 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Permeabilization
    Yes - 0.5% TritonX100
    Specification
    HeLa
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted May 26 2017

    Immunocytochemistry/ Immunofluorescence abreview for Anti-RanBP2 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 18°C
    Sample
    Human Cell (293T)
    Specification
    293T
    Permeabilization
    Yes - 0.5% Triton X100
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted May 15 2014

    Western blot abreview for Anti-RanBP2 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (4-12 Bis tris)
    Sample
    Human Cell lysate - whole cell (NALM6)
    Specification
    NALM6
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
    Read More

    Abcam user community

    Verified customer

    Submitted May 15 2014

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