Anti-RanBP2 antibody (ab64276)
- Datasheet
- References (16)
- Protocols
Overview
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Product name
Anti-RanBP2 antibody
See all RanBP2 primary antibodies -
Description
Rabbit polyclonal to RanBP2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cow
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Immunogen
Synthetic peptide (human) corresponding to 3 times repeated sequence in RanBP2 protein:
SKAPKSGFEGMFTKKE
, amino acids 1592-1607. -
Positive control
- HeLa whole cell lysate (ab150035) and HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: Whole serum -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab64276 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Detects a band of approximately 358 kDa (predicted molecular weight: 358 kDa). | |
| IP | Use at an assay dependent dilution. | |
| ICC/IF | 1/2000. |
Target
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Function
E3 SUMO-protein ligase which facilitates SUMO1 and SUMO2 conjugation by UBE2I. Involved in transport factor (Ran-GTP, karyopherin)-mediated protein import via the F-G repeat-containing domain which acts as a docking site for substrates. Could also have isomerase or chaperone activity and may bind RNA or DNA. Component of the nuclear export pathway. Specific docking site for the nuclear export factor exportin-1. -
Pathway
Protein modification; protein sumoylation. -
Involvement in disease
Defects in RANBP2 are the cause of susceptibility to encephalopathy acute necrotizing type 1 (ANE1) [MIM:608033]. A rapidly progressive encephalopathy manifesting in susceptibile individuals with seizures and coma. It can occur within days in otherwise healthy children after common viral infections such as influenza and parainfluenza, without evidence of viral infection of the brain or inflammatory cell infiltration. Brain T2-weighted magnetic resonance imaging reveals characteristic symmetric lesions present in the thalami, pons and brainstem. -
Sequence similarities
Contains 1 PPIase cyclophilin-type domain.
Contains 4 RanBD1 domains.
Contains 8 RanBP2-type zinc fingers.
Contains 1 TPR repeat. -
Domain
Contains F-X-F-G repeats. -
Post-translational
modificationsPolyubiquitinated by PARK2, which leads to proteasomal degradation. -
Cellular localization
Nucleus > nuclear pore complex. Cytoplasmic filaments. - Information by UniProt
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Database links
- Entrez Gene: 785768 Cow
- Entrez Gene: 5903 Human
- Omim: 601181 Human
- SwissProt: P48820 Cow
- SwissProt: P49792 Human
- Unigene: 199561 Human
- Unigene: 715056 Human
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Alternative names
- 358 kDa nucleoporin antibody
- ANE1 antibody
- E3 SUMO-protein ligase RanBP2 antibody
see all
Images
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Western blot - Anti-RanBP2 antibody (ab64276)Anti-RanBP2 antibody (ab64276) at 1/1000 dilution + HeLa total cell lysate
Predicted band size: 358 kDa
Observed band size: 358 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-RanBP2 antibody (ab64276)ab64276 (1:2000) staining RanBP2 in Hela cells pre-extracted in 0.005% digitonin and fixed in 3.7% PFA/30 mM sucrose/PBS for 15 min (room temperature). Image kindly provided by Patrizia Lavia, Univ. "La Sapienza", CNR, Italy. -
Immunoprecipitation - Anti-RanBP2 antibody (ab64276)RanBP2 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to RanBP2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab64276.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697) .
Band: 358kDa: RanBP2. -
Immunocytochemistry/ Immunofluorescence - Anti-RanBP2 antibody (ab64276)Immunofluorescent staining of paraformaldehyde fixed HeLa cells by ab64276 (1:2000). -
Western blot - Anti-RanBP2 antibody (ab64276)Anti-RanBP2 antibody (ab64276) at 1/2000 dilution + Hela whole cell lysate
Predicted band size: 358 kDa
Exposure time: 1 minute
WB performed on a 6%SDS-PAGE gel.Image Kindly provided by Patrizia Lavia, University "La Sapienza", CNR, Italy.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Bentz GL et al. Using glycyrrhizic acid to target sumoylation processes during Epstein-Barr virus latency. PLoS One 14:e0217578 (2019). Read more (PubMed: 31125383) »
- Liu S et al. Nuclear envelope assembly defects link mitotic errors to chromothripsis. Nature 561:551-555 (2018). Read more (PubMed: 30232450) »
