Anti-RanBP2 antibody (ab64276)
Key features and details
- Rabbit polyclonal to RanBP2
- Suitable for: WB, ICC/IF, IP
- Knockout validated
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-RanBP2 antibody
See all RanBP2 primary antibodies -
Description
Rabbit polyclonal to RanBP2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cow -
Immunogen
Synthetic peptide (human) corresponding to 3 times repeated sequence in RanBP2 protein:
SKAPKSGFEGMFTKKE
, amino acids 1592-1607. -
Positive control
- WB: Wild-type HeLa, HeLa and Caco2 whole cell lysates. ICC: HeLa cells.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6
Preservative: 0.09% Sodium azide
Constituent: Whole serum -
Concentration information loading...
-
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
KO cell lines
-
KO cell lysates
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab64276 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
1/1000. Detects a band of approximately 358 kDa (predicted molecular weight: 358 kDa).
|
ICC/IF | (3) |
1/2000.
|
IP |
Use at an assay dependent concentration.
|
Notes |
---|
WB
1/1000. Detects a band of approximately 358 kDa (predicted molecular weight: 358 kDa). |
ICC/IF
1/2000. |
IP
Use at an assay dependent concentration. |
Target
-
Function
E3 SUMO-protein ligase which facilitates SUMO1 and SUMO2 conjugation by UBE2I. Involved in transport factor (Ran-GTP, karyopherin)-mediated protein import via the F-G repeat-containing domain which acts as a docking site for substrates. Could also have isomerase or chaperone activity and may bind RNA or DNA. Component of the nuclear export pathway. Specific docking site for the nuclear export factor exportin-1. -
Pathway
Protein modification; protein sumoylation. -
Involvement in disease
Defects in RANBP2 are the cause of susceptibility to encephalopathy acute necrotizing type 1 (ANE1) [MIM:608033]. A rapidly progressive encephalopathy manifesting in susceptibile individuals with seizures and coma. It can occur within days in otherwise healthy children after common viral infections such as influenza and parainfluenza, without evidence of viral infection of the brain or inflammatory cell infiltration. Brain T2-weighted magnetic resonance imaging reveals characteristic symmetric lesions present in the thalami, pons and brainstem. -
Sequence similarities
Contains 1 PPIase cyclophilin-type domain.
Contains 4 RanBD1 domains.
Contains 8 RanBP2-type zinc fingers.
Contains 1 TPR repeat. -
Domain
Contains F-X-F-G repeats. -
Post-translational
modificationsPolyubiquitinated by PARK2, which leads to proteasomal degradation. -
Cellular localization
Nucleus > nuclear pore complex. Cytoplasmic filaments. - Information by UniProt
-
Database links
- Entrez Gene: 785768 Cow
- Entrez Gene: 5903 Human
- Omim: 601181 Human
- SwissProt: P48820 Cow
- SwissProt: P49792 Human
- Unigene: 199561 Human
- Unigene: 715056 Human
-
Alternative names
- 358 kDa nucleoporin antibody
- ANE1 antibody
- E3 SUMO-protein ligase RanBP2 antibody
see all
Images
-
All lanes : Anti-RanBP2 antibody (ab64276) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : RANBP2 knockout HeLa cell lysate
Lane 3 : Caco2 cell lysate
Lane 4 : MCF7 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 358 kDa
Observed band size: 450 kDa why is the actual band size different from the predicted?Lanes 1 - 4: Merged signal (red and green). Green - ab64276 observed at 450 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.
ab64276 was shown to react with RanBP2 in wild-type HeLa cells in Western blot with loss of signal observed in RANBP2 knockout cell line ab265618 (RANBP2 knockout cell lysate ab257627). Wild-type HeLa and RANBP2 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab64276 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 °C at a 1:1000 dilution and 1:20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1:20000 dilution for 1 h at room temperature before imaging.
-
Anti-RanBP2 antibody (ab64276) at 1/1000 dilution + HeLa total cell lysate
Predicted band size: 358 kDa
Observed band size: 358 kDa -
ab64276 (1:2000) staining RanBP2 in Hela cells pre-extracted in 0.005% digitonin and fixed in 3.7% PFA/30 mM sucrose/PBS for 15 min (room temperature). Image kindly provided by Patrizia Lavia, Univ. "La Sapienza", CNR, Italy.
-
Immunofluorescent staining of paraformaldehyde fixed HeLa cells by ab64276 (1:2000).
-
Anti-RanBP2 antibody (ab64276) at 1/2000 dilution + Hela whole cell lysate
Predicted band size: 358 kDa
Exposure time: 1 minute
WB performed on a 6%SDS-PAGE gel.Image Kindly provided by Patrizia Lavia, University "La Sapienza", CNR, Italy.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (29)
ab64276 has been referenced in 29 publications.
- Betancor G et al. MX2-mediated innate immunity against HIV-1 is regulated by serine phosphorylation. Nat Microbiol 6:1031-1042 (2021). PubMed: 34282309
- Agote-Arán A et al. Fragile X-Related Protein 1 Regulates Nucleoporin Localization in a Cell Cycle-Dependent Manner. Front Cell Dev Biol 9:755847 (2021). PubMed: 34977012
- Barbato S et al. Karyopherin enrichment at the nuclear pore complex attenuates Ran permeability. J Cell Sci 133:N/A (2020). PubMed: 31932502
- Dharan A et al. Nuclear pore blockade reveals that HIV-1 completes reverse transcription and uncoating in the nucleus. Nat Microbiol 5:1088-1095 (2020). PubMed: 32483230
- Marmor-Kollet H et al. Spatiotemporal Proteomic Analysis of Stress Granule Disassembly Using APEX Reveals Regulation by SUMOylation and Links to ALS Pathogenesis. Mol Cell 80:876-891.e6 (2020). PubMed: 33217318