Recombinant
RabMAb

Recombinant Anti-RanBP3 antibody [EPR5088(2)] (ab134052)

Overview

  • Product name

    Anti-RanBP3 antibody [EPR5088(2)]
    See all RanBP3 primary antibodies
  • Description

    Rabbit monoclonal [EPR5088(2)] to RanBP3
  • Host species

    Rabbit
  • Specificity

    The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse.

  • Tested applications

    Suitable for: WB, IP, IHC-P, ICC, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human RanBP3 aa 1-100. The exact sequence is proprietary.

  • Positive control

    • WB: HEK-293, MEF, and Y79 cell lysates; IHC: Human clear cell carcinoma of kidney, Human testis tissue.
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab134052 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 60 kDa).
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse.

ICC 1/100 - 1/250.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Acts as a cofactor for XPO1/CRM1-mediated nuclear export, perhaps as export complex scaffolding protein. Bound to XPO1/CRM1, stabilizes the XPO1/CRM1-cargo interaction. In the absence of Ran-bound GTP prevents binding of XPO1/CRM1 to the nuclear pore complex. Binds to CHC1/RCC1 and increases the guanine nucleotide exchange activity of CHC1/RCC1. Recruits XPO1/CRM1 to CHC1/RCC1 in a Ran-dependent manner. Negative regulator of TGF-beta signaling through interaction with the R-SMAD proteins, SMAD2 and SMAD3, and mediating their nuclear export.
  • Tissue specificity

    Widely expressed with high levels in testis and heart.
  • Sequence similarities

    Contains 1 RanBD1 domain.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • DKFZp586I1520 antibody
    • Ran binding protein 3 antibody
    • Ran-binding protein 3 antibody
    • RANB3_HUMAN antibody
    • RanBP3 antibody
    see all

Images

  • Immunohistochemistry of Human clear cell carcinoma of kidney tissues staining RanBP3 using ab134052 (1/1000). a HRP conjugated Goat Anti-Rabbit IgG H&L was used as a secondary antibody. 

    Antigen retrieval performed using heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    image shows nuclear and cytoplasmic staining on human clear cell carcinoma of kidney.

  • All lanes : Anti-RanBP3 antibody [EPR5088(2)] (ab134052) at 1/1000 dilution

    Lane 1 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates
    Lane 2 : MEF (Mouse embryonic fibroblast) whole cell lysates
    Lane 3 : Y79 (Human retinoblastoma retinoblastoma) whole cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/200000 dilution

    Predicted band size: 60 kDa
    Observed band size: 70,90 kDa
    why is the actual band size different from the predicted?


    Exposure time: 180 seconds


    The double bands are consistent with what has been described in PMID 16314428.

    We are unsure how to define the extra bands.

  • Immunohistochemical analysis of paraffin-embedded Human testis tissue labelling RanBP3 with ab134052 at 1/100 dilution.

References

ab134052 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab134052.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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