Overview

  • Product name
  • Description
    Goat polyclonal to RanBP9
  • Host species
    Goat
  • Tested applications
    Suitable for: IP, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Dog, Pig
  • Immunogen

    Synthetic peptide:

    GSCAFATVEDYLH

    , corresponding to C terminal amino acids 717-729 of Human RanBP9.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab5295 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration. PubMed: 21561988
ELISA Use at an assay dependent dilution. Peptide ELISA: Antibody detection limit dilution 1:16,000
IHC-P Use at an assay dependent concentration.

Target

  • Function
    May act as an adapter protein to couple membrane receptors to intracellular signaling pathways. May be involved in signaling of ITGB2/LFA-1 and other integrins. Enhances HGF-MET signaling by recruiting Sos and activating the Ras pathway. Involved in activation of androgen and glucocorticoid receptor in the presence of their cognate hormones. Stabilizes TP73 isoform Alpha, probably by inhibiting its ubiquitination, and increases its proapoptotic activity. Inhibits the kinase activity of DYRK1A and DYRK1B. Inhibits FMR1 binding to RNA.
  • Tissue specificity
    Ubiquitously expressed, with highest levels in testes, placenta, heart, and muscle, and lowest levels in lung. Within the brain, expressed predominantly by neurons in the gray matter of cortex, the granular layer of cerebellum and the Purkinje cells.
  • Sequence similarities
    Belongs to the RANBP9/10 family.
    Contains 1 B30.2/SPRY domain.
    Contains 1 CTLH domain.
    Contains 1 LisH domain.
  • Domain
    The SPRY domain mediates the interaction with MET, AR, and CDC2L1.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylated in response to stress. Can be phosphorylated by the cleaved p110 form of CDC2L1 (p110C).
    Ubiquitinated. Polyubiquitination targets the protein for rapid degradation via the ubiquitin system. Can be deubiquitinated by USP11.
  • Cellular localization
    Nucleus. Cytoplasm. A phosphorylated form is associated with the plasma membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • B cell antigen receptor Ig beta associated protein 1 antibody
    • BPM 90 antibody
    • BPM L antibody
    • BPM-L antibody
    • BPM90 antibody
    • BPML antibody
    • IBAP 1 antibody
    • Imp 9 antibody
    • Importin 9 antibody
    • Novel centrosomal protein RanBPM antibody
    • RAN binding protein 9 antibody
    • Ran binding protein centrosomal antibody
    • Ran Binding Protein in the Microtubule organizing center antibody
    • Ran binding protein M antibody
    • Ran BP9 antibody
    • Ran-binding protein 9 antibody
    • Ran-binding protein M antibody
    • RANB9_HUMAN antibody
    • RanBP 7 antibody
    • RANBP 9 antibody
    • RanBP7 antibody
    • RanBP9 antibody
    • RanBPM antibody
    see all

Images

  • Ab5295 (3ug/ml) staining of paraffin embedded Human Tonsil. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

References

This product has been referenced in:
  • Salemi LM  et al. Characterization of RanBPM molecular determinants that control its subcellular localization. PLoS One 10:e0117655 (2015). ICC/IF . Read more (PubMed: 25659156) »
  • Salemi LM  et al. Aggresome formation is regulated by RanBPM through an interaction with HDAC6. Biol Open 3:418-30 (2014). IF . Read more (PubMed: 24795145) »
See all 6 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Answer

Thank you for your enquiry. This antibody is a Fast-Track antibody. It has not been fully characterized in Western blot. As such, we cannot guarantee its use in Western blotting; however, you may be able to earn 1000 Abpoints for being the first scientist to give us conclusive information that this antibody works. Please see the datasheet (I have included a link below) for more details (the "Fast-Track antibody terms of use" link). The Abpoints may be redeemed for vouchers that will offset the purchase price of this antibody. A few recommendations: Did you incubated with this antibody overnight at 4C? If you incubated at room temperature, it is possible to further dilute the antibody and incubate longer with it to conserve antibody. Also, did you use a blocking agent in the primary solution? It is possible that the membrane is being overblocked, and that by eliminating the blocking agent in the primary, you will be able to detect your protein at a lower antibody concentration. In addition, in my lab we always trimmed the membrane and used parafilm to make a smaller vessel for incubating with the primary antibody (just slightly larger than the blot), and made up 10ml of solution. I have also heard of labs using "seal a meal" bags to incubate their blots, which allows you to use only a few mls of solution. I hope this information helps. Please do not hesitate to contact us if you need anything further.

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Question
Answer

Thank you for your enquiry. The synthetic peptide used to raise ab5295, anti-RanBP9 was GSCAFATVEDYLH, corresponding to C terminal amino acids 717-729 of Human RanBP9. Unfortunately the epitope of the antiserum has not been mapped. I hope this information helps.

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Answer

Thank you very much for contacting us regarding ab5295. I am very sorry to hear that you are experiencing difficulty with this antibody. As you may know, ab5295 is one of our Fast-track antibodies and it is not yet fully characterized. We cannot guarantee that the antibody will work in any application other than ELISA against the immunizing peptide and therefore we are unable to offer a refund if the antibody does not work in your application. However, Fast-Track antibodies are offered at a reduced price. We would be very grateful for you if you could submit a review via the product datasheet. We have a very special Feedback reward system for Fast-track products: we would greatly appreciate your feedback on this antibody, whether positive or negative. We will award 950 Abpoints to the first researcher who sends us positive feedback (including an image and details of materials & methods). Conclusive negative feedback that leads us to withdraw the antibody will also be rewarded with 950 Abpoints. Other useful positive and negative feedback will be rewarded with 50 Abpoints. To find out more about our Loyalty Scheme and Abpoints, please visit our website and look at Your Account panel (left bottom section on our home page) or the Earn Abpoints section on the product datasheet. All awards are subject to approval by Abcam scientists and provision of suitable data. Please submit feedback to fast-track@abcam.com and would be grateful if you could also attach your Western blot image. We hope to receive your review very shortly. Thank you for your time and your effort.

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Answer

Thank you for your enquiry. ab5295 is currently a fast-track antibody and has not been fully characterized. According to the datasheet there is a 76% identity between the immunogen and the corresponding rat homolog. I would therefore consider it highly possible that the result you are obtaining is a result of a failure of the antiserum to cross react against the rat RanBP9. However, there are several steps and controls that you could include to fully determine whether this is indeed the case. 1. Include a positive human (e.g. HeLa) cell lysate control (~20ug). 2. Try to use PVDF membrane. Generally, we find enhancement of transfer of proteins on PVDF membranes compared to nitroCellulose membranes. 3. Dilute the antibody 1:500 and incubate the filter overnight at 4 oC to see whether you are obtaining any signal whatsoever. 4. If you have enough material, try to load at least 20-30 ug protein/lane. Please let us know how you got on using this antibody by leaving an Abreview and in return we will award you 50 Abcam Points, which can be redeemed on a number of rewards (a further 100 Abcam Points will be offered for an image). Should you be the 1st researcher to leave positive or negative (conclusive) data in an Abreview for this product we will award you 1,000 Abcam points.

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Answer

Thank you for your enquiry. I'm afraid that I can't send our customer's blot to you, all of our customer information is confidential. As stated on the datasheet, ab5295 is a Fast-Track antibody; Fast-Track antibodies are immunogen affinity purified and are shown by ELISA to have high titre values against their immunogen. We are currently testing them for use in Western blotting. For more details, please see the Fast-Track antibody terms of use. So for ab5295, preliminary Western blot experiments gave no signal but low background in whole cell HeLa lysates at up to 1µg/ml. If you have any more questions, please contact us again.

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Answer

Thank you for getting back to us and for sharing your results with us. As you probably know, this is a Fast-track antibody and it has not been fully characterized. We have read through your protocol and would like to make the following suggestions/comments: 1. Try to use PVDF membrane. Generally, we find enhancement of transfer of proteins on PVDF membranes compared to nitrocellulose membranes. 2. We would also suggest diluting the antibody at 1:500 and incubating the filter overnight at 4 oC to see if the signal is getting stronger. 3. If you have enough material, try to load at least 20-30 ug protein/lane. Please do let us know how you are getting on with this antibody. We encourage customers to feed their results back to us, whether positive or negative, and make all information about each of our products available to researchers. In order to be able to send you a USD15/EUR15/GBP10 Amazon gift voucher, we would like to ask you to re-submit your review through the on-line datasheet which is the normal way for submitting feedback information. Even if you have already sent us your results to this e-mail address technical@abcam.com, we cannot resend your message as a review on-line - we have no right to do so. We look forward to hearing from you soon.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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