Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab5295 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration. PubMed: 21561988|
|ELISA||Use at an assay dependent concentration. Peptide ELISA: Antibody detection limit dilution 1:16,000|
|IHC-P||Use a concentration of 3 - 10 µg/ml.|
FunctionMay act as an adapter protein to couple membrane receptors to intracellular signaling pathways. May be involved in signaling of ITGB2/LFA-1 and other integrins. Enhances HGF-MET signaling by recruiting Sos and activating the Ras pathway. Involved in activation of androgen and glucocorticoid receptor in the presence of their cognate hormones. Stabilizes TP73 isoform Alpha, probably by inhibiting its ubiquitination, and increases its proapoptotic activity. Inhibits the kinase activity of DYRK1A and DYRK1B. Inhibits FMR1 binding to RNA.
Tissue specificityUbiquitously expressed, with highest levels in testes, placenta, heart, and muscle, and lowest levels in lung. Within the brain, expressed predominantly by neurons in the gray matter of cortex, the granular layer of cerebellum and the Purkinje cells.
Sequence similaritiesBelongs to the RANBP9/10 family.
Contains 1 B30.2/SPRY domain.
Contains 1 CTLH domain.
Contains 1 LisH domain.
DomainThe SPRY domain mediates the interaction with MET, AR, and CDC2L1.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylated in response to stress. Can be phosphorylated by the cleaved p110 form of CDC2L1 (p110C).
Ubiquitinated. Polyubiquitination targets the protein for rapid degradation via the ubiquitin system. Can be deubiquitinated by USP11.
Cellular localizationNucleus. Cytoplasm. A phosphorylated form is associated with the plasma membrane.
- Information by UniProt
- B cell antigen receptor Ig beta associated protein 1 antibody
- BPM 90 antibody
- BPM L antibody
This product has been referenced in:
- Salemi LM et al. Characterization of RanBPM molecular determinants that control its subcellular localization. PLoS One 10:e0117655 (2015). ICC/IF . Read more (PubMed: 25659156) »
- Salemi LM et al. Aggresome formation is regulated by RanBPM through an interaction with HDAC6. Biol Open 3:418-30 (2014). IF . Read more (PubMed: 24795145) »