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When we ued your product RanBP9 (cat# ab5295, order reference No. 97702; custmer No 2187139.), we could not see any bands on SDS-PAGE gel(Western blot and immunoprecipitation)when blotted with RanBP9 Ab, and could see some bands when blotted with other antibodies(it means that the gel is OK). The cell lysates used are from Hep2G(rat hepatocyte cell line), human hepatocyte cell line and human neuroblastoma cell line. It seems that anti human RanBP9 did not work. Could you show us what is positive control for anti RanBP9 blot and some image about positive bands? Thanks a lot. Looking forword to reply. Sincerely,
Asked on Oct 17 2005
Thank you very much for contacting us regarding ab5295. I am very sorry to hear that you are experiencing difficulty with this antibody. As you may know, ab5295 is one of our Fast-track antibodies and it is not yet fully characterized. We cannot guarantee that the antibody will work in any application other than ELISA against the immunizing peptide and therefore we are unable to offer a refund if the antibody does not work in your application. However, Fast-Track antibodies are offered at a reduced price. We would be very grateful for you if you could submit a review via the product datasheet. We have a very special Feedback reward system for Fast-track products: we would greatly appreciate your feedback on this antibody, whether positive or negative. We will award 950 Abpoints to the first researcher who sends us positive feedback (including an image and details of materials & methods). Conclusive negative feedback that leads us to withdraw the antibody will also be rewarded with 950 Abpoints. Other useful positive and negative feedback will be rewarded with 50 Abpoints. To find out more about our Loyalty Scheme and Abpoints, please visit our website and look at Your Account panel (left bottom section on our home page) or the Earn Abpoints section on the product datasheet. All awards are subject to approval by Abcam scientists and provision of suitable data. Please submit feedback to email@example.com and would be grateful if you could also attach your Western blot image. We hope to receive your review very shortly. Thank you for your time and your effort.
Answered on Oct 18 2005