Overview

  • Product name

    Anti-RanBP9 antibody [EPR9920(B)]
    See all RanBP9 primary antibodies
  • Description

    Rabbit monoclonal [EPR9920(B)] to RanBP9
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human RanBP9 aa 100-200. The exact sequence is proprietary.

  • Positive control

    • HeLa, fetal brain and JAR lysates; HeLa cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab140627 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 78 kDa.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function

      May act as an adapter protein to couple membrane receptors to intracellular signaling pathways. May be involved in signaling of ITGB2/LFA-1 and other integrins. Enhances HGF-MET signaling by recruiting Sos and activating the Ras pathway. Involved in activation of androgen and glucocorticoid receptor in the presence of their cognate hormones. Stabilizes TP73 isoform Alpha, probably by inhibiting its ubiquitination, and increases its proapoptotic activity. Inhibits the kinase activity of DYRK1A and DYRK1B. Inhibits FMR1 binding to RNA.
    • Tissue specificity

      Ubiquitously expressed, with highest levels in testes, placenta, heart, and muscle, and lowest levels in lung. Within the brain, expressed predominantly by neurons in the gray matter of cortex, the granular layer of cerebellum and the Purkinje cells.
    • Sequence similarities

      Belongs to the RANBP9/10 family.
      Contains 1 B30.2/SPRY domain.
      Contains 1 CTLH domain.
      Contains 1 LisH domain.
    • Domain

      The SPRY domain mediates the interaction with MET, AR, and CDC2L1.
    • Post-translational
      modifications

      Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylated in response to stress. Can be phosphorylated by the cleaved p110 form of CDC2L1 (p110C).
      Ubiquitinated. Polyubiquitination targets the protein for rapid degradation via the ubiquitin system. Can be deubiquitinated by USP11.
    • Cellular localization

      Nucleus. Cytoplasm. A phosphorylated form is associated with the plasma membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • B cell antigen receptor Ig beta associated protein 1 antibody
      • BPM 90 antibody
      • BPM L antibody
      • BPM-L antibody
      • BPM90 antibody
      • BPML antibody
      • IBAP 1 antibody
      • Imp 9 antibody
      • Importin 9 antibody
      • Novel centrosomal protein RanBPM antibody
      • RAN binding protein 9 antibody
      • Ran binding protein centrosomal antibody
      • Ran Binding Protein in the Microtubule organizing center antibody
      • Ran binding protein M antibody
      • Ran BP9 antibody
      • Ran-binding protein 9 antibody
      • Ran-binding protein M antibody
      • RANB9_HUMAN antibody
      • RanBP 7 antibody
      • RANBP 9 antibody
      • RanBP7 antibody
      • RanBP9 antibody
      • RanBPM antibody
      see all

    Images

    • All lanes : Anti-RanBP9 antibody [EPR9920(B)] (ab140627) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Fetal brain tissue lysate
      Lane 3 : JAR cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 78 kDa

    • Immunofluorescent staining of HeLa cells labelling RanBP9 with ab140627 at 1/100 dilution.

    References

    This product has been referenced in:

    • Bao J  et al. RAN-binding protein 9 is involved in alternative splicing and is critical for male germ cell development and male fertility. PLoS Genet 10:e1004825 (2014). Read more (PubMed: 25474150) »
    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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