Key features and details
- Rabbit polyclonal to RanGAP1
- Suitable for: IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-RanGAP1 antibody
See all RanGAP1 primary antibodies
DescriptionRabbit polyclonal to RanGAP1
Tested applicationsSuitable for: IP, IHC-Pmore details
Species reactivityReacts with: Human
Synthetic peptide within Human RanGAP1 aa 400-450. The exact sequence is proprietary. NP_002874.1
Database link: P46060
- IP: HEK-293T whole cell lysate. IHC-P: Human colon carcinoma tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab245544 was affinity purified using an epitope specific to RanGAP1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab245544 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
FunctionGTPase activator for the nuclear Ras-related regulatory protein Ran, converting it to the putatively inactive GDP-bound state.
Tissue specificityHighly expressed in brain, thymus and testis.
Sequence similaritiesBelongs to the RNA1 family.
Contains 6 LRR (leucine-rich) repeats.
modificationsPhosphorylated occurs before nuclear envelope breakdown and continues throughout mitosis. Phosphorylated by the M-phase kinase cyclin B/Cdk1, in vitro. Differential timimg of dephosphorylation occurs during phases of mitosis. The phosphorylated form remains associated with RANBP2/NUP358 and the SUMO E2-conjugating enzyme, UBC9, on nuclear pore complex (NPC) diassembly and during mitosis.
Sumoylated with SUMO1. Sumoylation is necessary for targeting to the nuclear envelope (NE), and for association with mitotic spindles and kinetochores during mitosis. Also required for interaction with RANBP2 and is mediated by UBC9.
Cellular localizationCytoplasm. Nucleus membrane. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle pole. Cytoplasmic during interphase. Targeted to the nuclear rim after sumoylation. During mitosis, associates with mitotic spindles. Association with kinetochores appears soon after nuclear envelope breakdown and persists until late anaphase. Mitotic location also requires sumoylation.
- Information by UniProt
- Fug 1 antibody
- Fug1 antibody
- GTPase-activating protein, RAN, 1 antibody
Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for RanGAP1 using ab245544 at 1/1000 dilution in immunohistochemical analysis. Detection: DAB staining.
RanGAP1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245544 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using a different Anti-RanGAP1 antibody at 1 µg/ml.
Lane 1: ab245544 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with an exposure time of 30 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab245544 has not yet been referenced specifically in any publications.