Anti-RanGAP1 antibody (ab245544)

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Overview

  • Product name

    Anti-RanGAP1 antibody
    See all RanGAP1 primary antibodies

  • Description

    Rabbit polyclonal to RanGAP1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, IHC-Pmore details
    Unsuitable for: WB

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human RanGAP1 aa 400-450. The exact sequence is proprietary. NP_002874.1
    Database link: P46060

  • Positive control

    • IP: HEK-293T whole cell lysate. IHC-P: Human colon carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab245544 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for WB.

    • Target

    • Function

      GTPase activator for the nuclear Ras-related regulatory protein Ran, converting it to the putatively inactive GDP-bound state.

    • Tissue specificity

      Highly expressed in brain, thymus and testis.

    • Sequence similarities

      Belongs to the RNA1 family.
      Contains 6 LRR (leucine-rich) repeats.

    • Post-translational
      modifications

      Phosphorylated occurs before nuclear envelope breakdown and continues throughout mitosis. Phosphorylated by the M-phase kinase cyclin B/Cdk1, in vitro. Differential timimg of dephosphorylation occurs during phases of mitosis. The phosphorylated form remains associated with RANBP2/NUP358 and the SUMO E2-conjugating enzyme, UBC9, on nuclear pore complex (NPC) diassembly and during mitosis.
      Sumoylated with SUMO1. Sumoylation is necessary for targeting to the nuclear envelope (NE), and for association with mitotic spindles and kinetochores during mitosis. Also required for interaction with RANBP2 and is mediated by UBC9.

    • Cellular localization

      Cytoplasm. Nucleus membrane. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle pole. Cytoplasmic during interphase. Targeted to the nuclear rim after sumoylation. During mitosis, associates with mitotic spindles. Association with kinetochores appears soon after nuclear envelope breakdown and persists until late anaphase. Mitotic location also requires sumoylation.
    • Information by UniProt

    • Database links

      • Alternative names

        • Fug 1 antibody
        • Fug1 antibody
        • GTPase-activating protein, RAN, 1 antibody
        • KIAA1835 antibody
        • MGC20266 antibody
        • OTTHUMP00000028918 antibody
        • OTTHUMP00000198755 antibody
        • OTTHUMP00000198756 antibody
        • OTTHUMP00000198757 antibody
        • OTTHUMP00000198758 antibody
        • RAGP1_HUMAN antibody
        • Ran 1 antibody
        • RAN GTPase activating protein 1 antibody
        • Ran GTPase-activating protein 1 antibody
        • Ran1 antibody
        • RANGAP 1 antibody
        • RANGAP antibody
        • RanGAP1 antibody
        • SD antibody
        • Segregation distorter homolog antibody
        • Segregation distortion antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RanGAP1 antibody (ab245544)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RanGAP1 antibody (ab245544)

        Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for RanGAP1 using ab245544 at 1/1000 dilution in immunohistochemical analysis. Detection: DAB staining.

      • Immunoprecipitation - Anti-RanGAP1 antibody (ab245544)
        Immunoprecipitation - Anti-RanGAP1 antibody (ab245544)

        RanGAP1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245544 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using a different Anti-RanGAP1 antibody at 1 µg/ml.

        Lane 1: ab245544 IP in HeLa whole cell lysate.
        Lane 2: Control IgG IP in HeLa whole cell lysate.

        Detection: Chemiluminescence with an exposure time of 30 seconds.

      References

      ab245544 has not yet been referenced specifically in any publications.

      Publishing research using ab245544? Please let us know so that we can cite the reference in this datasheet.

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