Product nameRanGAP1 overexpression 293T lysate (whole cell)
DescriptionRanGAP1 overexpression lysate
ab94064 is a 293T cell transfected lysate in which Human RanGAP1 has been transiently over-expressed using a pCMV-RanGAP1 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage bufferpH: 6.80
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol
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BackgroundFunction: GTPase activator for the nuclear Ras-related regulatory protein Ran, converting it to the putatively inactive GDP-bound state. PTM: Phosphorylated occurs before nuclear envelope breakdown and continues throughout mitosis. Phosphorylated by the M-phase kinase cyclin B/Cdk1, in vitro. Differential timimg of dephosphorylation occurs during phases of mitosis. The phosphorylated form remains associated with RANBP2/NUP358 and the SUMO E2-conjugating enzyme, UBC9, on nuclear pore complex (NPC) diassembly and during mitosis. Sumoylated with SUMO1. Sumoylation is necessary for targeting to the nuclear envelope (NE), and for association with mitotic spindles and kinetochores during mitosis. Also required for interaction with RANBP2 and is mediated by UBC9. Similarity: Belongs to the RNA1 family. Contains 6 LRR (leucine-rich) repeats. Tissue specificity: Highly expressed in brain, thymus and testis.
Our Abpromise guarantee covers the use of ab94064 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
ab94064 at 15µg/lane on an SDS-PAGE gel.
All lanes : Anti-RanGAP1 antibody (ab88682) at 1/500 dilution
Lane 1 : RanGAP1 293T Transfected Lysate - Positive Control (ab94064)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
All lanes : non-Abcam, Goat Anti-mouse IgG (H and L) HRP conjugated, at 1/2500 dilution
ab94064 has not yet been referenced specifically in any publications.